抗生素的微生物转化：天蓝色链霉菌Müller对克林霉素的磷酸化作用。
Microbial transformation of antibiotics: phosphorylation of clindamycin by Streptomyces coelicolor Müller.
作者信息
Coats J H, Argoudelis A D
出版信息
J Bacteriol. 1971 Oct;108(1):459-64. doi: 10.1128/jb.108.1.459-464.1971.
Abstract
Addition of clindamycin to whole-cell cultures of Streptomyces coelicolor Müller resulted in the loss of in vitro activity against organisms sensitive to clindamycin. Incubation of such culture filtrates with alkaline phosphatase generated a biologically active material identified as clindamycin. Fermentation broths containing inactivated clindamycin yielded clindamycin 3-phosphate, the structure of which was established by physical-chemical and enzymatic studies. Clindamycin was phosphorylated by lysates and partially purified enzyme preparations from S. coelicolor Müller. These reactions require a ribonucleoside triphosphate and Mg(2+). The product of the cell-free reactions was identified as clindamycin 3-phosphate.
摘要
向天蓝色链霉菌Müller的全细胞培养物中添加克林霉素会导致其对克林霉素敏感的生物体失去体外活性。将此类培养滤液与碱性磷酸酶一起孵育会产生一种被鉴定为克林霉素的生物活性物质。含有失活克林霉素的发酵液产生了克林霉素3-磷酸酯，其结构通过物理化学和酶学研究得以确定。克林霉素可被天蓝色链霉菌Müller的裂解物和部分纯化的酶制剂磷酸化。这些反应需要三磷酸核糖核苷和Mg(2+)。无细胞反应的产物被鉴定为克林霉素3-磷酸酯。
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