不动杆菌临床分离株的血清分型：基因种3的血清型

Serotyping of clinical isolates of Acinetobacter: serovars of genospecies 3.

作者信息

Traub W H

机构信息

Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg.

出版信息

Zentralbl Bakteriol. 1990 May;273(1):12-23. doi: 10.1016/s0934-8840(11)80235-5.

DOI:10.1016/s0934-8840(11)80235-5

PMID:2363794

Abstract

A total of 156 clinical isolates of genospecies 3, the second most commonly encountered member of the genus Acinetobacter at our institution, were identified with carbon source utilization tests among 400 Acinetobacter isolates. Checkerboard tube agglutination tests and reciprocal cross-absorption studies with polyclonal rabbit immune sera against heated (100 degrees C, 1 h) cells of serovar candidate strains of genospecies 3 permitted identification of 13 serovars. Minor one-way crossreactions were noted between serovars 10 and 8 and between serovars 2 and 12, respectively. Isolates of serovar 13 cross-reacted extensively one-way with serovar 2 and less so with serovars 4 and 12. Genospecies 3 serovars 3, 7, 8, and 9 cross-reacted with Acinetobacter baumannii serovars 19, 21, 6, and 15, respectively.

摘要

在400株不动杆菌分离株中，通过碳源利用试验共鉴定出156株基因型3的临床分离株，该基因型是我院第二常见的不动杆菌属成员。棋盘式试管凝集试验以及使用针对基因型3血清型候选菌株加热（100℃，1小时）细胞的兔多克隆免疫血清进行的双向交叉吸收研究，鉴定出了13个血清型。血清型10和8之间以及血清型2和12之间分别观察到轻微的单向交叉反应。血清型13的分离株与血清型2广泛单向交叉反应，与血清型4和12的交叉反应较少。基因型3的血清型3、7、8和9分别与鲍曼不动杆菌的血清型19、21、6和15交叉反应。