Traub W H, Leonhard B, Bauer D
Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg/Saar, Germany.
Zentralbl Bakteriol. 1996 Jun;284(1):115-23. doi: 10.1016/s0934-8840(96)80160-5.
Triplets of isolates representing 20 putative clusters of nosocomial cross-infection due to Acinetobacter baumannii and genospecies 3 were examined comparatively using serotyping and analysis of restriction fragments (SmaI and ApaI) of genomic DNA with the aid of pulsed-field gel electrophoresis. Carbon source assimilation tests disclosed phenotypic variation among 6 to 20 triplets of isolates. Two misleading results of serotyping were encountered. With respect to the presumptive cluster No. 9, one of the genospecies 3 (originally serovar 4) isolates proved to be polyagglutinable upon repeat examination; this particular putative cluster was shown to be a pseudocluster by comparison of the macrorestriction profiles of the respective triple isolates. A strain of A. baumannii serovar 15 had infected 8 patients in a surgical intensive care unit, while a second, genotypically totally different strain of identical serovar had caused infection in one additional patient. With this exception, the correlation between serotyping and analysis of macrorestriction profiles was excellent.
使用血清分型以及借助脉冲场凝胶电泳对基因组DNA的限制性片段（SmaI和ApaI）进行分析，对代表由鲍曼不动杆菌和基因种3引起的20个假定医院交叉感染集群的三联分离株进行了比较研究。碳源同化试验揭示了6至20个三联分离株之间的表型差异。遇到了两个血清分型的误导性结果。关于假定的第9集群，其中一个基因种3（原血清型4）分离株在重复检测时被证明具有多凝集性；通过比较相应三联分离株的宏观限制性图谱，表明这个特定的假定集群是一个假集群。一株血清型15的鲍曼不动杆菌在一个外科重症监护病房感染了8名患者，而另一株血清型相同但基因型完全不同的菌株在另外一名患者中引起了感染。除了这个例外，血清分型与宏观限制性图谱分析之间的相关性非常好。
