Department of Molecular Microbiology and Biotechnology, Life Sciences, Tel Aviv University, Tel Aviv, Israel.
PLoS One. 2013 Apr 29;8(4):e62297. doi: 10.1371/journal.pone.0062297. Print 2013.
A new group of translation-associated heat shock genes has been recently identified. One of these novel genes is ybeY which is highly conserved in bacteria. In Escherichia coli the YbeY protein is important for efficient translation at all temperatures and is essential at high temperatures. Deletion mutants of ybeY are defective in protein translation, due to impaired 30 S ribosomal subunits. Here we provide evidence which tie YbeY to the transcription antitermination process. Thus, in ybeY deletion mutants transcription is significantly inhibited when the "nut like" sequences required for transcriptional antitermination are present, while if these sequences are removed transcription is not affected by the mutation.
最近发现了一组新的与翻译相关的热休克基因。这些新基因中的一个是 ybeY，它在细菌中高度保守。在大肠杆菌中，YbeY 蛋白在所有温度下对高效翻译都很重要，在高温下是必需的。ybeY 缺失突变体在蛋白质翻译中存在缺陷，这是由于 30S 核糖体亚基受损所致。在这里，我们提供的证据将 YbeY 与转录终止抑制过程联系起来。因此，在 ybeY 缺失突变体中，如果存在转录终止抑制所需的“螺母样”序列，转录会受到显著抑制，而如果这些序列被去除，突变不会影响转录。
