乙酰肝素酶-1在激光诱导脉络膜新生血管形成中的作用
Heparanase-1 activities in the development of laser induced choroidal neovascularization.
作者信息
Tang Wei-Qiang, Hou Bao-Ke
机构信息
Department of Ophthalmology, the First Affiliated Hospital of General Hospital of People's Liberation Army, Beijing 100048, China.
出版信息
Int J Ophthalmol. 2013 Apr 18;6(2):131-5. doi: 10.3980/j.issn.2222-3959.2013.02.04. Print 2013.
AIM
To investigate the role of heparanase-1 in laser-induced choroidal neovascularization (CNV).
METHODS
Experimental CNV was induced by krypton laser photocoagulation in 15 male Brown Norway rats. Fundus fluorescein angiography and histopathological examination were performed in observing the CNV development. The expression and distribution of heparanase-1 protein in the laser lesions were determined by immunohistochemistry and western blotting analysis.
RESULTS
The success rate of laser induced CNV was approximately 75% on 3-4 weeks after laser photocoagulation. The protein levels of heparanase-1 increased significantly in the retina-choroidal complex of CNV models when compared to normal rat eyes (P<0.01). Immunostaining confirmed strong heparanase-1 expressions in all laser lesions, and it displayed to be highest at the newly formed blood vessels within the fibrovascular complex in the subretinal space.
CONCLUSION
Heparanase-1 is closely involved in the development of laser induced CNV.
目的
研究乙酰肝素酶-1在激光诱导脉络膜新生血管（CNV）中的作用。
方法
对15只雄性挪威棕色大鼠进行氪激光光凝诱导实验性CNV。通过眼底荧光血管造影和组织病理学检查观察CNV的发展。采用免疫组织化学和蛋白质印迹分析确定激光损伤部位乙酰肝素酶-1蛋白的表达和分布。
结果
激光光凝后3-4周，激光诱导CNV的成功率约为75%。与正常大鼠眼相比，CNV模型视网膜-脉络膜复合体中乙酰肝素酶-1的蛋白水平显著升高（P<0.01）。免疫染色证实所有激光损伤部位均有强烈的乙酰肝素酶-1表达，且在视网膜下间隙纤维血管复合体中的新生血管处表达最高。
结论
乙酰肝素酶-1密切参与激光诱导CNV的发展。
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