Wang Chao, Han Hongyan, Gu Xueying, Yu Zhu, Nishino Naoki
Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
Anim Sci J. 2014 Jan;85(1):32-6. doi: 10.1111/asj.12076. Epub 2013 May 3.
To evaluate the current practice of corn silage management in China, samples of bunker-made silage were collected from 14 farms within a 500-km radius of Beijing for the analysis of fermentation products and bacterial communities. Mean values for dry matter (DM) content were as low as 250 g/kg in both corn stover (St) and whole crop corn (Wc) silages, and pH values averaged 4.48 and 3.73, respectively. Only three of the 14 silages exhibited a lactic-to-acetic acid ratio > 1.0, indicating that the presence of acetic acid was predominant in fermentation. Although 1,2-propanediol content was marginal in most cases (< 5.0 g/kg dry matter (DM)), two Wc silages had 1,2-propanediol levels > 25 g/kg DM. In contrast, 3 St silages had large amounts (> 10 g/kg DM) of butyric acid, and two of the three butyrate silages also had high concentrations of 1-propanol. Denaturing gradient gel electrophoresis analysis demonstrated that the bacterial community appeared similar in 10 out of the 14 silage samples. Bands indicating Lactobacillus buchneri, L. acetotolerans and Acetobacter pasteurianus were found in both the St and Wc silages, accounting for the high acetic acid content found across silage samples.
为评估中国目前玉米青贮饲料的管理实践,从北京半径500公里范围内的14个农场采集了青贮窖制作的青贮饲料样本,用于分析发酵产物和细菌群落。玉米秸秆(St)青贮饲料和全株玉米(Wc)青贮饲料的干物质(DM)含量平均值均低至250克/千克,pH值分别平均为4.48和3.73。14个青贮饲料样本中只有3个样本的乳酸与乙酸比率大于1.0,这表明发酵过程中乙酸占主导地位。尽管在大多数情况下1,2 - 丙二醇含量较低(<5.0克/千克干物质(DM)),但有两个Wc青贮饲料样本的1,2 - 丙二醇含量>25克/千克DM。相比之下,有3个St青贮饲料样本含有大量(>10克/千克DM)丁酸,这3个含丁酸盐的青贮饲料样本中有2个还含有高浓度的1 - 丙醇。变性梯度凝胶电泳分析表明,14个青贮饲料样本中有10个样本的细菌群落相似。在St和Wc青贮饲料样本中均发现了指示布氏乳杆菌、耐乙酸乳杆菌和巴氏醋杆菌的条带,这解释了青贮饲料样本中乙酸含量较高的原因。