Department of Animal Science, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo CEP 14884-900, Brazil.
Department of Animal Science, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, São Paulo CEP 14884-900, Brazil; Agência Paulista de Tecnologia dos Agronegócios, Polo Regional Alta Mogiana, Colina, São Paulo CEP 14770-000, Brazil.
J Dairy Sci. 2018 May;101(5):4158-4167. doi: 10.3168/jds.2017-13797. Epub 2018 Feb 14.
We investigated the effects of different types and doses of inoculants for ensiling rehydrated corn grain. Shelled corn was finely ground and rehydrated to 35% moisture. Treatments were as follows: (1) control (no additives); (2) Lactobacillus plantarum and Pediococcus acidilactici (LPPA) at a theoretical application rate of 1 × 10 cfu/g; (3) LPPA at 5 × 10 cfu/g; (4) LPPA at 1 × 10 cfu/g; (5) Lactobacillus buchneri (LB) at 1 × 10 cfu/g; (6) LB at 5 × 10 cfu/g; and (7) LB at 1 × 10 cfu/g. We detected no effect of inoculant dose. Gas losses were greater in silages treated with LB compared with control and LPPA silages. Treating silages with LB reduced the concentrations of lactic acid and ethanol and increased silage pH and concentrations of acetic acid, propionic acid, and 1,2-propanediol. At silo opening, silages treated with LB had higher counts of lactic acid bacteria but lower yeast counts than the control silage. Aerobic stability was greater for silages treated with LB and lower for silages treated with LPPA compared with the control. The LB reduced dry matter (DM) losses during aerobic exposure, whereas LPPA increased them. Prolamin content was lower in silages treated with LB compared with the control, resulting in greater ruminal in situ DM degradability. Inoculating LB to a dose of 1 × 10 cfu/g increased aerobic stability and ruminal in situ DM degradability of rehydrated corn grain silage. The addition of LPPA did not alter the fermentation process and worsened the aerobic stability of rehydrated corn grain silage. Further studies are warranted to confirm these conclusions in other corn hybrids, inoculants, and their combinations.
我们研究了不同类型和剂量的接种剂对再水合玉米谷物青贮的影响。去壳玉米被精细地研磨并再水合至 35%的水分。处理如下：(1)对照（无添加剂）；(2)植物乳杆菌和戊糖片球菌（LPPA），理论应用率为 1×10 cfu/g；(3)LPPA 为 5×10 cfu/g；(4)LPPA 为 1×10 cfu/g；(5)布氏乳杆菌（LB）为 1×10 cfu/g；(6)LB 为 5×10 cfu/g；和(7)LB 为 1×10 cfu/g。我们没有检测到接种剂量的影响。与对照和 LPPA 青贮相比，用 LB 处理的青贮中的气体损失更大。用 LB 处理青贮降低了乳酸和乙醇的浓度，增加了青贮的 pH 值以及乙酸、丙酸和 1,2-丙二醇的浓度。在青贮开窖时，用 LB 处理的青贮中乳酸菌的数量高于对照青贮，但酵母菌的数量低于对照青贮。与对照相比，用 LB 处理的青贮具有更高的好氧稳定性，而用 LPPA 处理的青贮则更低。LB 减少了好氧暴露过程中的干物质（DM）损失，而 LPPA 则增加了损失。用 LB 处理的青贮中 prolamin 含量低于对照，导致瘤胃原位 DM 降解率更高。用 1×10 cfu/g 的剂量接种 LB 增加了再水合玉米谷物青贮的好氧稳定性和瘤胃原位 DM 降解率。添加 LPPA 没有改变发酵过程，反而恶化了再水合玉米谷物青贮的好氧稳定性。需要进一步的研究来确认这些结论在其他玉米杂交种、接种剂及其组合中的适用性。
