College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
Anim Reprod Sci. 2013 Jun;139(1-4):95-100. doi: 10.1016/j.anireprosci.2013.03.015. Epub 2013 Apr 6.
The aim of the present study was to evaluate the cryoprotective effect of Laminaria japonic polysaccharide (LJP) on boar sperm. Semen samples were collected from seven mature Yorkshire boars once a week by the gloved hand technique and frozen-thawed in the extender with LJP added. Extender with LJP added at concentrations of 0.25, 0.5, 1.0, 1.5 and 2.0mg/mL to the extender and its effects on the quality of frozen-thawed boar sperm were assessed. Results showed: (i) sperm motility and plasma membrane integrity were greater in the extender containing 0.5 and 1.0mg/mL LJP, as compared to other groups (P<0.05); (ii) extender added 1.0mg/mL LJP showed the greatest plasma membrane and acrosomal integrity percentages in comparison with other groups (P<0.05); (iii) mitochondrial activity was significantly higher at the concentration of 0.5 and 1.0mg/mL LJP than those of other groups (P<0.05); (iv) in terms of biochemical assessments, 0.5 and 1.0mg/mL LJP improved SOD (superoxide dismutase) and CAT (catalase) concentrations, compared to other groups (P<0.05). However, no significant difference was found in GSH-Px (glutathione peroxidase) concentration when supplemented with LJP. Interestingly, LJP exhibited a dose-related response and the lesser concentration represented greater protective effects. It is also important to note that 1.0mg/mL LJP provides for an enhanced cryoprotective effect in boar semen.
本研究旨在评估海带多糖（LJP）对猪精子的抗冷冻保护作用。每周通过手套法从 7 头成熟约克夏公猪中收集一次精液样本，并在添加 LJP 的稀释液中进行冷冻-解冻。将添加浓度为 0.25、0.5、1.0、1.5 和 2.0mg/mL 的 LJP 的稀释液添加到稀释液中，并评估其对冷冻-解冻猪精子质量的影响。结果表明：（i）与其他组相比，含有 0.5 和 1.0mg/mL LJP 的稀释液中精子活力和质膜完整性更高（P<0.05）；（ii）与其他组相比，添加 1.0mg/mL LJP 的稀释液显示出更高的质膜和顶体完整性百分比（P<0.05）；（iii）在 0.5 和 1.0mg/mL LJP 的浓度下，线粒体活性明显高于其他组（P<0.05）；（iv）与其他组相比，0.5 和 1.0mg/mL LJP 提高了 SOD（超氧化物歧化酶）和 CAT（过氧化氢酶）的浓度（P<0.05）。然而，添加 LJP 时 GSH-Px（谷胱甘肽过氧化物酶）浓度没有显著差异。有趣的是，LJP 表现出剂量相关的反应，较低的浓度代表更好的保护效果。同样重要的是要注意，1.0mg/mL LJP 可提高猪精液的抗冷冻保护作用。
