Zheng Yuxin, Zhang Nina, Liu Shujie, Li Qingwang, Jiang Zhongliang
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China.
State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinhai University, Xining, Qinghai 810016, China.
Cryobiology. 2017 Dec;79:50-55. doi: 10.1016/j.cryobiol.2017.09.002. Epub 2017 Sep 20.
In this study, water-soluble Laminaria japonica polysaccharide3 (LJP-P3) was investigated for the cryoprotective effects on bull sperm. Five concentrations of LJP-P3 with 0.1, 1, 10, 50 and 100 mmol/L were added into the extenders of bull semen, respectively, and the effects on quality of sperm after freezing-thawing were assessed. The results showed that the kinematic parameters of bull sperm including linear motile sperm (LM), curvilinear line velocity (VCL) value, straight line velocity (VSL) and velocity of the average path (VAP) were greater in the extenders containing LJP-P3 (P<0.05). In comparison to those of other treatments and control group the extenders containing 1.0, 10.0 and 50.0 mmol/L of LJP-P3 led to higher percentage of mitochondrial activity and sperm membrane integrity(P<0.05), and the acrosome integrity of bull cryopreservation sperm were significantly improved in all treatment groups. Moreover, the higher GSH-Px, SOD and CAT levels in bull cryopreservation sperm were favored from the extenders of 10.0, 50.0 and 100.0 mmol/L LJP-P3 added (P<0.05) compared with other treatments and control group. In addition, the results of artificial insemination showed that both the pregnancy rate and the number of calving were higher in the group of semen containing 10 mmol/L of LJP-P3 than that of control group (P <0.05). In summary, LJP-P3 exhibited a greater cryoprotective effect to bull sperm and the most suitable concentration of LJP-P3 is 10.0 mmol/L.
在本研究中，对水溶性海带多糖3（LJP-P3）对公牛精子的冷冻保护作用进行了研究。分别将0.1、1、10、50和100 mmol/L的五种浓度的LJP-P3添加到公牛精液稀释液中，并评估其对冻融后精子质量的影响。结果表明，在含有LJP-P3的稀释液中，公牛精子的运动学参数包括直线运动精子（LM）、曲线速度（VCL）值、直线速度（VSL）和平均路径速度（VAP）更高（P<0.05）。与其他处理组和对照组相比，含有1.0、10.0和50.0 mmol/L LJP-P3的稀释液导致线粒体活性和精子膜完整性的百分比更高（P<0.05），并且在所有处理组中，公牛冷冻保存精子的顶体完整性均得到显著改善。此外，与其他处理组和对照组相比，添加10.0、50.0和100.0 mmol/L LJP-P3的稀释液有利于提高公牛冷冻保存精子中更高的谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平（P<0.05）。此外，人工授精结果表明，含有10 mmol/L LJP-P3的精液组的妊娠率和产犊数均高于对照组（P<0.05）。综上所述，LJP-P3对公牛精子表现出更大的冷冻保护作用，LJP-P3的最适浓度为10.0 mmol/L。
