在电子断层扫描中确定手性的挑战，以及 DNA 折纸金纳米粒子螺旋作为分子标准的应用。
The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards.
机构信息
Division of Biology, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.
出版信息
J Struct Biol. 2013 Jul;183(1):95-8. doi: 10.1016/j.jsb.2013.04.008. Epub 2013 Apr 30.
Abstract
The apparent handedness of an EM-tomography reconstruction depends on a number of conventions and can be confused in many ways. As the number of different hardware and software combinations being used for electron tomography continue to climb, and the reconstructions being produced reach higher and higher resolutions, the need to verify the hand of the results has increased. Here we enumerate various steps in a typical tomography experiment that affect handedness and show that DNA origami gold nanoparticle helices can be used as convenient and fail-safe handedness standards.
摘要
EM 断层重建的明显手性取决于许多约定，并且可能在许多方面混淆。随着用于电子断层重建的不同硬件和软件组合的数量不断增加，并且生成的重建达到越来越高的分辨率，对手性结果进行验证的需求也在增加。在这里，我们列举了典型断层重建实验中影响手性的各个步骤，并表明 DNA 折纸金纳米粒子螺旋可以用作方便且可靠的手性标准。
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