Guo T Y, Xia Y Q, Hao G J, Song M D, Zhang B H
State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Weijin Road 94, Tianjin China, Tianjin 300071 PR China.
Biomaterials. 2004 Dec;25(27):5905-12. doi: 10.1016/j.biomaterials.2004.01.032.
A simply hemoglobin (Hb) molecularly imprinted polymer (MIP) was prepared using Hb as the imprinted molecule, acrylamide as the functional monomer and cross-linked chitosan beads as the supporting matrix. The MIP was achieved by entrapment of the selective soft polyacrylamide gel in the pores of the cross-linked chitosan beads by letting acrylamide monomer and the protein diffuse into the pores of chitosan beads before starting the polymerization. The chitosan beads were freed from the surrounding polyacrylamide gel by washing. The Langmuir and Freundlich adsorption models were applied to describe the equilibrium isotherms. Langmuir analysis showed that an equal class of adsorption was formed in the MIP and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. The MIP has much higher adsorption capacity for Hb than the non-imprinted polymer with the same chemical composition, and the MIP also has a higher selectivity for the imprinted molecule. The MIP can be reused in an easy way and the reproduction coefficient was approximately 100% at low concentration.
以血红蛋白（Hb）为印迹分子、丙烯酰胺为功能单体、交联壳聚糖珠为支撑基质，制备了一种简单的血红蛋白分子印迹聚合物（MIP）。通过在聚合反应开始前让丙烯酰胺单体和蛋白质扩散到壳聚糖珠的孔中，将选择性软聚丙烯酰胺凝胶包埋在交联壳聚糖珠的孔中来制备MIP。通过洗涤使壳聚糖珠与周围的聚丙烯酰胺凝胶分离。应用朗缪尔和弗伦德里希吸附模型来描述平衡等温线。朗缪尔分析表明，MIP中形成了同等类型的吸附，并评估了吸附平衡常数和最大吸附容量。与具有相同化学组成的非印迹聚合物相比，MIP对Hb具有更高的吸附容量，并且MIP对印迹分子也具有更高的选择性。MIP可以很容易地重复使用，并且在低浓度下再生系数约为100%。
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