Maize Research Institute, Department of Technology and Breeding Department, Slobodana Bajića 1, 11085, Belgrade-Zemun, Serbia.
J Sci Food Agric. 2014 Jan 15;94(1):45-51. doi: 10.1002/jsfa.6210. Epub 2013 Jun 7.
The Maillard reaction indicators furosine, hydroxymethylfurfural (HMF), acrylamide and color were determined to evaluate heat effects induced during extrusion, infrared and microwave heating of soybean. In addition, the present paper aimed to study changes in the phenolic compounds, as well as in the overall antioxidant properties of different soybean products in relation to heating at 45-140 °C during the processes.
Soybean proteins were highly sensible to Maillard reaction and furosine was rapidly formed under slight heating conditions during extrusion and infrared heating. Microwave heating at lower temperatures for a longer time yielded lower acrylamide levels in the final soybean products, as a result of its partial degradation. However, during infrared heating, acrylamide formation greatly increased with decreasing moisture content. After a short time of extrusion and infrared heating at 140 °C and microwave heating at 135 °C for 5 min, concentrations of HMF increased to 11.34, 26.21 and 34.97 µg g(-1), respectively.
The heating conditions caused formation of acrylamide, HMF and furosine in high concentration. The results indicate that the complex structure of soybeans provides protection of phenolic compounds from thermal degradation, and that Maillard reaction products improved the antioxidant properties of heat-treated soybean.
本研究通过检测美拉德反应指标——呋咱、羟甲基糠醛(HMF)、丙烯酰胺和色泽,来评估挤压、红外和微波加热对大豆产生的热效应。此外,本研究还旨在研究不同大豆制品在 45-140°C 加热过程中酚类化合物和总体抗氧化性能的变化。
大豆蛋白极易发生美拉德反应,在挤压和红外加热的轻微加热条件下,呋咱迅速形成。在较低温度下长时间进行微波加热会使最终大豆制品中的丙烯酰胺水平降低,这是由于其部分降解。然而,在红外加热过程中,随着水分含量的降低,丙烯酰胺的形成大大增加。在挤压和红外加热 140°C 以及微波加热 135°C 5 分钟后,HMF 的浓度分别增加到 11.34、26.21 和 34.97 µg g(-1)。
加热条件导致丙烯酰胺、HMF 和呋咱的形成浓度较高。结果表明,大豆的复杂结构为酚类化合物提供了免受热降解的保护,且美拉德反应产物提高了热处理大豆的抗氧化性能。