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五指山猪不同组织中Myf5和MyoD1基因的表达与变异

Expression and variation of Myf5 and MyoD1 genes in different tissues of Wuzhishan pigs.

作者信息

Hou G Y, Zhou H L, Cao T, Xun W J, Wang D J, Shi L G, Guan S, Wang D F, Li M

机构信息

Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan, China

Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan, China.

出版信息

Genet Mol Res. 2015 Apr 17;14(2):3729-35. doi: 10.4238/2015.April.17.22.

DOI:10.4238/2015.April.17.22
PMID:25966141
Abstract

The myogenic regulatory factor (MRF) family includes Myf5, MyoD1, Myf4, and Mfy6 genes. This experiment assessed the variation of Myf5 and MyoD1 genes from birth to maturity (30, 210, and 360 days) in the back muscle tissue of Wuzhishan pigs (WZSP), and the expression of Myf5 and MyoD1 mRNA in the heart, liver, lung, spleen, kidney, muscle, stomach, and intestine tissues were also examined. The results indicate that the expression level of mRNA for Myf5 and MyoD1 genes in the back muscle tissue is directly proportional to age (P < 0.05). Furthermore, of the eight adult pig tissue types that were tested, the expression of Myf5 and MyoD1 was highest in the muscle tissue.

摘要

生肌调节因子(MRF)家族包括Myf5、MyoD1、Myf4和Mfy6基因。本实验评估了五指山猪(WZSP)背肌组织从出生到成熟(30、210和360天)期间Myf5和MyoD1基因的变化,同时检测了Myf5和MyoD1 mRNA在心脏、肝脏、肺、脾脏、肾脏、肌肉、胃和肠道组织中的表达。结果表明,背肌组织中Myf5和MyoD1基因的mRNA表达水平与年龄成正比(P < 0.05)。此外,在测试的八种成年猪组织类型中,Myf5和MyoD1在肌肉组织中的表达最高。

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