S.I.P.R.E-Comité Nord, rue des champs Potez, Achicourt, France.
J Sep Sci. 2013 Jul;36(14):2379-85. doi: 10.1002/jssc.201300188.
The aim of this investigation was the preparative isolation of solanidine (aglycone of the two main potato glycoalkaloids: α-chaconine and α-solanine) from fresh Solanum tuberosum (cv. Pompadour) material by implementing a new preparation scheme using centrifugal partition chromatography (CPC). A setup for obtaining solanidine by hydrolysis of the glycoalkaloids found in the skin and sprouts of S. tuberosum was first developed. Then its isolation was carried out by the development of CPC conditions: the solvent system used for separation was ethyl acetate/butanol/water in the ratio 42.5:7.5:50 v/v/v, 0.6 g of crude extract were separated with a 8 mL/min flow rate of mobile phase while rotating at 2500 rpm. A run yielded 98 mg of solanidine (86.7% recovery from the crude extract) in a one-step separation. The purity of the isolated solanidine was over 98%. Thus, CPC has proven to be the method of choice to get solanidine of very high purity from S. tuberosum biomass in large quantities.
本研究旨在通过实施一种新的制备方案，利用离心分配色谱（CPC）从新鲜的马铃薯（品种为 Pompadour）材料中分离茄啶（两种主要马铃薯糖苷生物碱：α-茄碱和α-龙葵碱的糖苷配基）。首先开发了一种从马铃薯的皮和芽中发现的糖苷生物碱水解获得茄啶的方法。然后通过开发 CPC 条件来进行分离：分离中使用的溶剂系统为乙酸乙酯/正丁醇/水，体积比为 42.5:7.5:50v/v/v，以 8mL/min 的流速在 2500rpm 下旋转，用 0.6g 粗提取物进行分离。一步分离可得到 98mg 茄啶（从粗提取物中回收 86.7%）。分离得到的茄啶纯度超过 98%。因此，CPC 已被证明是从大量马铃薯生物量中获得高纯度茄啶的首选方法。
