Toyoda H, Himeno S, Imura N
Department of Public Health, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Biochim Biophys Acta. 1990 Jun 21;1049(2):213-5. doi: 10.1016/0167-4781(90)90042-z.
Glutathione peroxidase (GSH-Px) contains selenium at its active site as a selenocysteine moiety. We have shown that feeding mice a selenium-deficient diet for a long period caused a large decrease in the GSH-Px mRNA level as well as in GSH-Px activity both in the liver and kidneys (Toyoda, H., Himeno, S. and Imura, N. (1989) Biochim. Biophys. Acta 1008, 301-308). In the present study, the transcription rate of the GSH-Px gene was determined by a nuclear run-on assay using liver nuclei of mice fed a selenium-deficient or selenium-adequate diet. The results clearly demonstrate that the transcription rate of the GSH-Px gene was not changed by dietary selenium manipulation, indicating that dietary selenium regulates the level of GSH-Px mRNA in the post-transcriptional step.
谷胱甘肽过氧化物酶（GSH-Px）在其活性位点含有作为硒代半胱氨酸部分的硒。我们已经表明，长期给小鼠喂食缺硒饮食会导致肝脏和肾脏中GSH-Px mRNA水平以及GSH-Px活性大幅下降（丰田博、日野诚、井村直，（1989年）《生物化学与生物物理学学报》1008，301 - 308）。在本研究中，使用喂食缺硒或富硒饮食的小鼠肝脏细胞核，通过核转录分析测定GSH-Px基因的转录速率。结果清楚地表明，饮食中硒的控制并未改变GSH-Px基因的转录速率，这表明饮食中的硒在转录后步骤调节GSH-Px mRNA的水平。
