Zhao Minmeng, Li Jiahui, Shi Qiao, Shan Haoshu, Liu Long, Geng Tuoyu, Yu Lei, Gong Daoqing
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, 225009, People's Republic of China.
Zhenjiang Animal Disease Prevention and Control Center, Zhenjiang, 212009, People's Republic of China.
Biol Trace Elem Res. 2023 Dec;201(12):5764-5773. doi: 10.1007/s12011-023-03611-5. Epub 2023 Mar 10.
This study aims to investigate the impacts of in ovo feeding (IOF) of selenized glucose (SeGlu) on selenium (Se) level and antioxidant capacity of breast muscle in newborn broilers. After candling on 16 day of incubation, a total of 450 eggs were randomly divided into three treatments. On the 17.5th day of incubation, eggs in a control treatment were injected with 0.1 mL of physiological saline (0.75%), while the 2nd group and 3rd group were supplied with 0.1 mL of physiological saline containing 10 μg Se from SeGlu (SeGlu group) and 20 μg Se from SeGlu (SeGlu group). The results showed that in ovo injection in both SeGlu and SeGlu increased the Se level and reduced glutathione concentration (GSH) in pectoral muscle of hatchlings (P < 0.05). Compared with the control group, the SeGlu-treated chicks significantly enhanced the activity of the superoxide dismutase (SOD) and mRNA expression of NAD(P)H quinone dehydrogenase 1 (NQO1) in breast muscle, while there was upregulation in mRNA expressions of glutathione peroxidase 1 (GPX-1) and thioredoxin reductase 1 (TrxR1) and higher total antioxidant capacity (T-AOC) in SeGlu treatment (P < 0.05). However, no significant difference on enzyme activities of glutathione peroxidase (GR), glutathione reductase, thioredoxin reductase, concentration of malondialdehyde, and free radical scavenging ability (FRSA) of superoxide radical (O) and hydroxyl radical (OH) was observed among the three treatments (P > 0.05). Therefore, IOF of SeGlu enhanced Se deposition in breast muscle of neonatal broilers. In addition, in ovo injection of SeGlu could increase the antioxidant capacity of newborn chicks possibly through upregulating the mRNA expression of GPX1, TrxR1, and NQO1, as well as the SOD activity.
本研究旨在探讨卵内注射（IOF）亚硒酸钠葡萄糖（SeGlu）对新生肉鸡胸肌中硒（Se）水平和抗氧化能力的影响。在孵化第16天进行照蛋后，将总共450枚鸡蛋随机分为三个处理组。在孵化第17.5天，对照组鸡蛋注射0.1 mL生理盐水（0.75%），而第二组和第三组分别注射含10 μg Se的0.1 mL SeGlu生理盐水（SeGlu组）和含20 μg Se的0.1 mL SeGlu生理盐水（SeGlu组）。结果表明，SeGlu和SeGlu组的卵内注射均提高了雏鸡胸肌中的硒水平并降低了谷胱甘肽浓度（GSH）（P < 0.05）。与对照组相比，SeGlu处理的雏鸡显著提高了胸肌中超氧化物歧化酶（SOD）的活性以及NAD（P）H醌脱氢酶1（NQO1）的mRNA表达，而SeGlu处理组中谷胱甘肽过氧化物酶1（GPX-1）和硫氧还蛋白还原酶1（TrxR1）的mRNA表达上调且总抗氧化能力（T-AOC）更高（P < 0.05）。然而，在三种处理组之间，谷胱甘肽过氧化物酶（GR）、谷胱甘肽还原酶、硫氧还蛋白还原酶的酶活性、丙二醛浓度以及超氧阴离子（O）和羟自由基（OH）的自由基清除能力（FRSA）均未观察到显著差异（P > 0.05）。因此，SeGlu的卵内注射增强了新生肉鸡胸肌中的硒沉积。此外，卵内注射SeGlu可能通过上调GPX1、TrxR1和NQO1的mRNA表达以及SOD活性来提高新生雏鸡的抗氧化能力。
