Mikuska L, Vrabcova M, Lackovicova L, Ukropec J, Hegedusova N, Slavkovsky P, Hubka P, Mravec B
Slovak Academy of Sciences, Bratislava, Slovak Republic.
Endocr Regul. 2013 Apr;47(2):85-92. doi: 10.4149/endo_2013_02_85.
Obesity is particularly associated with an increased consumption of palatable fat and sugar dense food and beverages. Therefore, we examined the effect of a normocaloric liquid diet (Fresubin) with increased carbohydrate content (constituting 55% of calories) on development of obesity in different developmental periods in male Wistar rats.
Fresubin was provided to 3 groups of rats: the first group received Fresubin immediately after weaning (21st day of age) to the end of experiment (150th day of age) for 5 months; the second group was fed with Fresubin from weaning to adulthood (90th day of age) for 3 months; and the third group received Fresubin only in adulthood (from 90th to 150th day of age) for 2 months. The control group was fed with standard pellet chow from weaning to the end of the experiment. Body weight, food and water intake were periodically measured. After terminating the experiment, the adiposity index was determined.
Rats fed with liquid nutrition showed increased energy intake and body weight in comparison with the control rats. Interestingly, while obesity in the juvenile rats developed as late as of 13 weeks after the Fresubin intake, the adult rats fed with liquid nutrition had significantly elevated the body weight already 2 weeks after starting the treatment. Increased adiposity index was observed in both groups of rats fed with Fresubin during the whole study as well as the adulthood.
Our data indicate that feeding of male Wistar rats with a high carbohydrate normocaloric diet results in a substantial development of obesity. Moreover, exposure of juvenile individuals to obesogenic environment leads, after a certain "latent period", to the development of obesity that may reflect low protein content of used liquid diet or higher resistance of juvenile organism to the obesogenic factors. Finally, based on the data obtained we suggest that Fresubin, with respect to its properties, may serve as a diet for the development of obesity which may exemplify an "obesity model" applicable in small laboratory animals.
肥胖尤其与美味的高脂肪和高糖食品及饮料的摄入量增加有关。因此，我们研究了一种碳水化合物含量增加（占热量的55%）的等热量液体饮食（Fresubin）对雄性Wistar大鼠不同发育阶段肥胖发生发展的影响。
将Fresubin提供给3组大鼠：第一组在断奶后（21日龄）立即给予Fresubin直至实验结束（150日龄），持续5个月；第二组从断奶到成年期（90日龄）给予Fresubin，持续3个月；第三组仅在成年期（90至150日龄）给予Fresubin，持续2个月。对照组从断奶到实验结束给予标准颗粒饲料。定期测量体重、食物和水的摄入量。实验结束后，测定肥胖指数。
与对照大鼠相比，喂食液体营养的大鼠能量摄入量和体重增加。有趣的是，虽然幼鼠在摄入Fresubin后13周才出现肥胖，但喂食液体营养的成年大鼠在开始治疗后2周体重就显著升高。在整个研究期间以及成年期，两组喂食Fresubin的大鼠均观察到肥胖指数增加。
我们的数据表明，用高碳水化合物等热量饮食喂养雄性Wistar大鼠会导致肥胖的大量发生。此外，幼年个体暴露于致肥胖环境中，经过一定的“潜伏期”后会导致肥胖的发生，这可能反映了所用液体饮食中蛋白质含量低或幼年生物体对致肥胖因素的抵抗力较高。最后，根据获得的数据，我们认为Fresubin就其特性而言，可作为一种导致肥胖的饮食，它可能是适用于小型实验动物的“肥胖模型”。
