Department of Agrobioscience and ‡Organization of Advanced Science and Technology, Kobe University , 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
J Agric Food Chem. 2013 May 22;61(20):4850-4. doi: 10.1021/jf305322t. Epub 2013 May 13.
Simple pharmacological studies on inositol stereoisomers are presented in this study. Male ICR mice were orally administered 1 g/kg BW of three inositol stereoisomers, myo-inositol (MI), d-chiro-inositol (DCI), and scyllo-inositol (SI), and blood plasma samples and skeletal muscle fractions were prepared after an hour. The plasma samples were subjected to gas chromatography-coupled time-of-flight mass spectrometry (GC-TOF-MS) analysis. None of the three stereoisomers was seen in untreated samples, but substantial amounts ranging from 2.5 to 6.5 mM were detected only after administration, indicating that orally administered inositol stereoisomers were readily absorbed and their levels elevated in the bloodstream. In addition, plasma of SI-administered animals contained substantial MI, suggesting a possible metabolic conversion of SI to MI. In the skeletal muscle fractions, glucose transporter type 4 (GLUT4) content in the plasma membrane increased, indicating that inositol stereoisomers stimulated GLUT4 translocation.
本研究对肌醇立体异构体进行了简单的药理学研究。雄性 ICR 小鼠经口给予 1 g/kg BW 的三种肌醇立体异构体（肌醇（MI）、d-手性肌醇（DCI）和 scyllo-肌醇（SI）），1 小时后制备血浆样品和骨骼肌部分。将血浆样品进行气相色谱-飞行时间质谱（GC-TOF-MS）分析。未处理的样品中未检测到这三种立体异构体，但给药后仅检测到 2.5 至 6.5 mM 的大量物质，表明口服给予的肌醇立体异构体很容易被吸收，其水平在血液中升高。此外，SI 给药动物的血浆中含有大量的 MI，提示 SI 可能发生了向 MI 的代谢转化。在骨骼肌部分，质膜中的葡萄糖转运蛋白 4（GLUT4）含量增加，表明肌醇立体异构体刺激 GLUT4 易位。
