Department of Chemical Engineering, Loughborough University, Loughborough, UK.
J Hosp Infect. 2013 Jun;84(2):181-3. doi: 10.1016/j.jhin.2013.02.020. Epub 2013 May 3.
Spores of Clostridium difficile were deposited on to a stainless steel surface and subsequently exposed to a chlorine-releasing disinfectant (dichloroisocyanurate). Recovery of the spores was carried out using RODAC plates containing a variety of selective and non-selective agars. The non-selective agar media yielded higher recoveries of both control and chlorine-stressed spores. Our results show that the antibiotics used in selective media imposed an additional stress on both disinfectant-treated and untreated spores resulting in considerably reduced recoveries. This could lead to a serious underestimate of the extent of environmental contamination by this organism.
艰难梭菌的孢子被沉积在不锈钢表面上，然后暴露于释放氯的消毒剂（二氯异氰尿酸酯）中。使用含有各种选择性和非选择性琼脂的 RODAC 平板进行孢子回收。非选择性琼脂培养基对对照和氯胁迫孢子的回收率均更高。我们的结果表明，选择性培养基中使用的抗生素对消毒剂处理和未处理的孢子施加了额外的压力，导致回收率大大降低。这可能导致对该生物体的环境污染程度的严重低估。
