Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.
Mol Phylogenet Evol. 2013 Sep;68(3):381-6. doi: 10.1016/j.ympev.2013.04.017. Epub 2013 May 3.
The hawkmoth genus Manduca is a diverse group of very large, conspicuous moths that has served as an important model across many biological disciplines. Two species in particular, the tobacco hornworm (Manduca sexta) and the tomato hornworm (Manduca quinquemaculatus) have been researched extensively. Studies across biological fields have referred to these two species as being closely related or even sister species, but the extent to which these two model organisms are related remains largely unknown. We conducted a comprehensive multi-gene phylogenetic analysis of Manduca, based on both an ML and Bayesian framework, which resulted in a monophyletic Manduca but only when two other genera, Dolba and Euryglottis are included. We tentatively conclude that the sister group to Manduca sexta comprises the Caribbean M. afflicta and M. johanni, and the sister lineage to this clade includes M. quinquemaculatus and the Hawaiian M. blackburni. Thus, M. sexta and M. quinquemaculatus are closely related, but are not sister species. Biogeographical analyses reveal an ancestral center of diversification in Central America, and Manduca appears to have subsequently colonized North and South America. Our phylogeny provides an important foundation for comparative studies of two model organisms and their relatives.
烟草天蛾属（Manduca）是一个多样化的大型、显著蛾类群，在许多生物学领域都被用作重要的模式生物。其中两个特别的物种，烟草夜蛾（Manduca sexta）和番茄天蛾（Manduca quinquemaculatus）已经被广泛研究。跨生物学领域的研究将这两个物种称为密切相关甚至是姐妹种，但这两个模式生物之间的亲缘关系程度在很大程度上仍然未知。我们基于最大似然法和贝叶斯框架对 Manduca 进行了全面的多基因系统发育分析，结果表明 Manduca 是单系的，但只有当包括另外两个属 Dolba 和 Euryglottis 时才会如此。我们初步得出结论，与烟草夜蛾亲缘关系最近的是加勒比 Manduca afflicta 和 M. johanni，而这个分支的姊妹谱系包括番茄天蛾和夏威夷 Manduca blackburni。因此，烟草夜蛾和番茄天蛾密切相关，但不是姐妹种。生物地理分析显示中美洲是多样化的祖先中心，而 Manduca 似乎随后在北美和南美进行了殖民化。我们的系统发育为两个模式生物及其亲属的比较研究提供了重要的基础。
