人及牛牙釉质中糖蛋白、脂蛋白和磷蛋白的电泳显示
Electrophoretic demonstration of glycoproteins, lipoproteins, and phosphoproteins in human and bovine enamel.
作者信息
Kirkeby S, Moe D, Bøg-Hansen T C, Salling E
机构信息
Institute of General and Oral Anatomy, Royal Dental College of Copenhagen, Denmark.
出版信息
Caries Res. 1990;24(3):169-73. doi: 10.1159/000261261.
Enamel proteins from fully mineralized human molars and from bovine tooth germs were separated by electrophoresis. The gels were stained for detection of glycoproteins, lipoproteins, and phosphoproteins. Glycoproteins were shown by periodic acid-Schiff staining and lectin blotting. In mature human enamel a number of high molecular weight proteins could be demonstrated after ethylenediaminetetra-acetic acid demineralization and subsequent Triton X-100 extraction. These proteins are suggested to be lipoproteins. Phosphoproteins could only be visualized in enamel matrix from the tooth germs.
通过电泳分离来自完全矿化的人类磨牙和牛牙胚的牙釉质蛋白。对凝胶进行染色以检测糖蛋白、脂蛋白和磷蛋白。通过过碘酸希夫染色和凝集素印迹法显示糖蛋白。在成熟的人类牙釉质中，经乙二胺四乙酸脱矿质处理并随后用 Triton X - 100 提取后，可以证明有一些高分子量蛋白质。这些蛋白质被认为是脂蛋白。磷蛋白仅在牙胚的牙釉质基质中可见。
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