School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Bioresour Technol. 2013 Oct;146:44-50. doi: 10.1016/j.biortech.2013.07.046. Epub 2013 Jul 18.
Ammonium removal from source water is usually inhibited by insufficient carbon sources and low temperature in Northeastern China. A strain Y16 was isolated from oligotrophic niche and was identified as Acinetobacter sp. Y16. It demonstrated excellent capability for ammonium removal at 2 °C, and simultaneously produced nitrogen gas as the end product. About 66% of ammonium was removed after 36 h of incubation. Only trace accumulation of nitrate was observed during the process. The utilization of nitrite and nitrate as well as the existence of napA gene further proved the aerobic denitrification ability of strain Y16. Sodium acetate was the most favorable carbon source for ammonium oxidation by strain Y16. High rotation speed was beneficial for ammonium oxidation. Furthermore, strain Y16 could efficiently remove ammonium at low C/N ratio and low temperature conditions, which was advantageous for nitrogen removal from source water under cold temperatures.
从水源中去除氨氮通常受到东北地区碳源不足和低温的抑制。从贫营养生境中分离出一株菌 Y16，鉴定为不动杆菌 sp. Y16。该菌在 2°C 时具有良好的氨氮去除能力，同时将氮气作为终产物。经过 36 小时的培养，约有 66%的氨氮被去除。在整个过程中仅观察到痕量硝酸盐的积累。亚硝酸盐和硝酸盐的利用以及 napA 基因的存在进一步证明了菌株 Y16 的好氧反硝化能力。乙酸钠是菌株 Y16 进行氨氧化作用的最适碳源。高转速有利于氨氮的氧化。此外，菌株 Y16 可以在低 C/N 比和低温条件下有效地去除氨氮，这有利于在低温下从水源中去除氮。
