Infertility Solutions, Allentown, PA 18103, USA.
J Assist Reprod Genet. 2013 Jun;30(6):855-60. doi: 10.1007/s10815-013-0006-1. Epub 2013 May 5.
This study compared the impact of using the Steiner-Tan pseudo double lumen needle for antral follicle oocyte retrieval to using a conventional non-flushing needle. The Steiner-Tan needle has a much smaller dead space than the needles commonly used for IVM oocyte retrievals.
This was a retrospective cohort study. The patient population was determined by the time period in which a patient underwent IVM in a single physician's IVF practice. The following data was abstracted from clinical and embryology records: oocytes retrieved, oocytes matured, early maturing oocytes, oocytes fertilized, embryo quality measures, retrieval time, needle punctures, clot formation, and clinical pregnancy rate.
The Steiner-Tan needle did not increase the number of oocytes retrieved. It also did not increase the time required for retrieval. However, flushing of antral follicles significantly decreased clot formation in fluid aspirates. Use of the Steiner-Tan needle also significantly decreased the number of vaginal needle punctures during each case. There was a trend toward improved embryo quality, but statistical power was inadequate to show a difference.
The primary benefit of the Steiner-Tan needle was on the embryological aspects of IVM. Decreased blood and blood clots in the aspirates made an IVM retrieval more like conventional IVF for the embryologist. The patient also experienced less tissue trauma without increasing anesthesia or surgical time. There was no improvement in the number of oocytes retrieved, but based on the results, we hypothesized that oocytes were more commonly retrieved from slightly large follicles than when using a routine needle.
本研究比较了使用 Steiner-Tan 假双腔针进行窦卵泡卵母细胞采集与使用传统非冲洗针的效果。Steiner-Tan 针的死腔比常用于 IVM 卵母细胞采集的针小得多。
这是一项回顾性队列研究。患者人群由在一位医生的 IVF 实践中进行 IVM 的时间段确定。从临床和胚胎学记录中提取以下数据：采集的卵母细胞、成熟的卵母细胞、早期成熟的卵母细胞、受精的卵母细胞、胚胎质量指标、采集时间、针穿刺次数、凝块形成和临床妊娠率。
Steiner-Tan 针并未增加采集的卵母细胞数量，也未增加采集所需的时间。然而，窦卵泡冲洗显著减少了抽吸液中的凝块形成。使用 Steiner-Tan 针也显著减少了每个病例中阴道针穿刺的次数。胚胎质量有改善的趋势，但统计能力不足以显示差异。
Steiner-Tan 针的主要益处在于 IVM 的胚胎学方面。抽吸液中血液和血凝块的减少使 IVM 采集更类似于常规 IVF，这对胚胎学家来说更为方便。患者也经历了较少的组织创伤，而不会增加麻醉或手术时间。采集的卵母细胞数量没有增加，但根据结果，我们假设卵母细胞更常从稍大的卵泡中采集，而不是使用常规针。
