College of Agriculture, Liaocheng University, Liaocheng, 252059, Shandong, People's Republic of China.
Mol Biol Rep. 2013 Aug;40(8):4675-83. doi: 10.1007/s11033-013-2562-y. Epub 2013 May 6.
Recently, variations in or near Lin28B gene were reported to be associated with timing of human puberty by genome-wide association studies. There have been no reports on that in other mammals. In the present study, a fragment of 5,353 bp of goat Lin28B cDNA, encoding 247 amino acids, was amplified, which contains 744 bp coding region and 4,410 bp 3'-untranslated region (UTR). Two alternative transcripts of Lin28BS (encoding 247 aa) and Lin28BL (encoding 261 aa) were found. Eight mutations in 3'-UTR were detected in nine goat breeds, and the T allele frequencies of A2934T and C3053T in the five sexual precocious breeds were higher than that of the four sexual late-maturing breeds (p<0.05). This may indicate that both the 2934 and 3053 locus may be associated with the age of goat puberty. Our results might expand understanding of the biological pathway of Lin28B gene regulating the timing of mammal puberty.
最近，全基因组关联研究报道 Lin28B 基因的变异或附近区域与人类青春期的时间有关。在其他哺乳动物中尚未有相关报道。本研究扩增了山羊 Lin28B cDNA 的 5353bp 片段，编码 247 个氨基酸，其中包含 744bp 编码区和 4410bp 3'-非翻译区（UTR）。发现了 Lin28BS（编码 247 个氨基酸）和 Lin28BL（编码 261 个氨基酸）两种替代转录本。在 9 个山羊品种中检测到 3'-UTR 中的 8 个突变，在 5 个性早熟品种中，A2934T 和 C3053T 的 T 等位基因频率高于 4 个性晚熟品种（p<0.05）。这可能表明 2934 和 3053 位点都可能与山羊青春期的年龄有关。我们的研究结果可能会扩展对 Lin28B 基因调节哺乳动物青春期时间的生物学途径的理解。
