College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China.
J Zhejiang Univ Sci B. 2013 May;14(5):382-91. doi: 10.1631/jzus.B1200317.
Skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs in plastic surgery. The mechanisms of I/R injury include hypoxia, inflammation, and oxidative damage. Hydrogen gas has been reported to alleviate cerebral I/R injury by acting as a free radical scavenger. Here, we assessed the protective effect of hydrogen-rich saline (HRS) on skin flap I/R injury.
Abdominal skin flaps of rats were elevated and ischemia was induced for 3 h; subsequently, HRS or physiological saline was administered intraperitoneally 10 min before reperfusion. On postoperative Day 5, flap survival, blood perfusion, the accumulation of reactive oxygen species (ROS), and levels of cytokines were evaluated. Histological examinations were performed to assess inflammatory cell infiltration.
Skin flap survival and blood flow perfusion were improved by HRS relative to the controls. The production of malondialdehyde (MDA), an indicator of lipid peroxidation, was markedly reduced. A multiplex cytokine assay revealed that HRS reduced the elevation in the levels of inflammatory cytokines, chemokines and growth factors, with the exception of RANTES (regulated on activation, normal T-cell expressed and secreted) growth factor. HRS treatment also reduced inflammatory cell infiltration induced by I/R injury.
Our findings suggest that HRS mitigates I/R injury by decreasing inflammation and, therefore, has the potential for application as a therapy for improving skin flap survival.
缺血再灌注（I/R）引起的皮肤损伤是一个多因素的过程，这种情况在整形手术中经常发生。I/R 损伤的机制包括缺氧、炎症和氧化损伤。有报道称，氢气可以作为自由基清除剂来减轻脑 I/R 损伤。在这里，我们评估了富氢生理盐水（HRS）对皮瓣 I/R 损伤的保护作用。
大鼠腹部皮瓣被抬高并缺血 3 小时；然后，在再灌注前 10 分钟通过腹腔内给予 HRS 或生理盐水。在术后第 5 天，评估皮瓣存活率、血液灌注、活性氧（ROS）的积累和细胞因子水平。进行组织学检查以评估炎症细胞浸润。
与对照组相比，HRS 提高了皮瓣存活率和血流灌注。丙二醛（MDA）的产生，一种脂质过氧化的指标，明显减少。多重细胞因子检测显示，HRS 降低了炎症细胞因子、趋化因子和生长因子水平的升高，除 RANTES（调节激活正常 T 细胞表达和分泌）生长因子外。HRS 治疗还减轻了 I/R 损伤引起的炎症细胞浸润。
我们的研究结果表明，HRS 通过减少炎症来减轻 I/R 损伤，因此具有作为改善皮瓣存活的治疗方法的潜力。
