Shimode K, Fujihara S, Nakamura M, Kobayashi S, Tsunematsu T
Third Division of Internal Medicine, Shimane Medical University.
Rinsho Shinkeigaku. 1990 Mar;30(3):288-93.
The lower level of cystatin C in cerebrospinal fluid (CSF) is one of the useful diagnostic markers of hereditary cerebral hemorrhage with amyloidosis in Iceland. We attempted to establish an assay to determine the level of cystatin C in CSF for diagnosis of CAA due to the deposition of cystatin C in CSF for diagnosis of CAA due to the deposition of cystatin C. We carried out the sandwich enzyme immunosorbent assay with the use of monoclonal mouse anti-cystatin C and polyclonal rabbit anti-cystatin C antibodies. CSF from nine cases of cerebral hemorrhage and fifty reference cases with other neurological diseases were examined. Four patients with cerebral hemorrhage showed a low level of cystatin C and clinical manifestations suggestive of CAA. Our study showed the feasibility of using ELISA for the diagnosis of cerebral amyloid angiopathy that causes cerebral hemorrhage with the deposition of cystatin C.
脑脊液中胱抑素C水平较低是冰岛遗传性淀粉样脑血管病脑出血的有用诊断标志物之一。由于胱抑素C沉积导致的脑淀粉样血管病（CAA），我们试图建立一种测定脑脊液中胱抑素C水平的检测方法用于CAA的诊断。我们使用单克隆小鼠抗胱抑素C抗体和多克隆兔抗胱抑素C抗体进行夹心酶免疫吸附测定。检测了9例脑出血患者的脑脊液以及50例患有其他神经系统疾病的对照病例的脑脊液。4例脑出血患者显示胱抑素C水平较低且有提示CAA的临床表现。我们的研究表明，使用酶联免疫吸附测定法诊断因胱抑素C沉积导致脑出血的脑淀粉样血管病是可行的。
