Department of Chemistry, W. M. Keck Institute for Proteomics Technology and Applications, The George Washington University, Washington, DC 20052, USA.
Analyst. 2013 Jun 21;138(12):3444-9. doi: 10.1039/c3an00631j. Epub 2013 May 3.
Direct mass spectrometric analysis of animal tissues is an emerging field enabled by recent developments in ambient ion sources. Label-free in situ analysis of metabolites, lipids, and peptides/proteins from intact tissues in whole fish specimens of different gender and age were performed by laser ablation electrospray ionization (LAESI) mass spectrometry (MS). Hypertrophied glandular tissue (gill gland) of adult male Aphyocharax anisitsi (bloodfin tetra) was compared with gill tissues in females of the same species. Comparison of a large number of sample-specific ions was aided by a multivariate statistical method based on orthogonal projections to latent structures discriminant analysis. More than 200 different ions were detected in the mass spectra corresponding to primary metabolites, hormones, lipids and peptides/proteins. The gill tissues of the sexually mature males exhibited multiply charged ions in the 6+ to 10+ charge states corresponding to a protein with a molecular weight of 11 380 Da. This protein was present only in the mature male gill glands but absent in the corresponding area of the female and immature male specimens. An additional nine proteins were detected by LAESI-MS in both the male and female gill tissues.
直接质谱分析动物组织是一个新兴领域，得益于环境离子源的最新发展。激光烧蚀电喷雾电离（LAESI）质谱（MS）可对不同性别和年龄的完整鱼标本中的代谢物、脂质和肽/蛋白质进行无标记原位分析。与同物种的雌性鱼相比，对成年雄性 Aphyocharax anisitsi（血鳍 Tetra）的肥大腺组织（鳃腺）进行了比较。通过基于正交投影到潜结构判别分析的多元统计方法，对大量特定于样本的离子进行了比较。在对应于初级代谢物、激素、脂质和肽/蛋白质的质谱中检测到了 200 多种不同的离子。性成熟雄性的鳃组织表现出 6+到 10+电荷状态的多电荷离子，对应于分子量为 11380 Da 的蛋白质。这种蛋白质仅存在于成熟雄性的鳃腺中，而在雌性和未成熟雄性标本的相应区域不存在。LAESI-MS 在雄性和雌性鳃组织中还检测到另外 9 种蛋白质。
