Tu Yunjie, Su Yijun, Li Guohui, Zhang Xueyu, Tong Haibing
Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, 225125, China.
J Poult Sci. 2016 Apr 25;53(2):118-123. doi: 10.2141/jpsa.0140071.
Differential lipid metabolic requirements of sexually-mature males and females may influence the regulation of lipid metabolism-associated genes and hence the content of adipose tissue. We measured the expression of eight lipid metabolism-associated genes (fatty acid synthase, ; acylglycerol- 3- phosphate O-acyltransferase 9, ; peroxisomal proliferator-activated receptor ; lipoprotein lipase, ; carnitine palmitoyl transferase 1 A, ; carnitine palmitoyl transferase 1 B, ; acyl-COA dehydrogenase long chain, ; monoglyceride lipase, ) in eight tissues (hypothalamus, HYP; liver; heart; pectoralis major muscle, PM; gastrocnemius muscle, GAS; abdominal fat, AF; clavicular fat, CF; subcutaneous fat, SF) of five male and five female white feather chickens using real time PCR at 217 d (when the females were at peak egg production). There were no difference between sexes, nor were there sex by tissue interactions for and . In both cases expression was greater for liver than the other tissues. When interactions of sex by tissue were significant, the mRNA abundance in HYP, liver, and PM was greater for females than males. There was no sexual dimorphism for any tissue for . Overall values were greater for adipose depots than HYP and liver with muscles intermediate for . mRNA abundance in PM and AF was greater for females than males, with the pattern reversed for heart and SF. mRNA abundance in GAS and CF was greater for females than males, with the relationship reversed for liver. mRNA abundance in HYP, liver, and GAS was greater for females than males, and lower in PM than males. The results demonstrated that expression of lipid metablism-associated genes varies among sexes in mature chickens depending on the gene and the tissue.
性成熟的雄性和雌性对脂质代谢的不同需求可能会影响脂质代谢相关基因的调控，进而影响脂肪组织的含量。我们在217日龄（此时雌性处于产蛋高峰期）时，使用实时PCR技术测量了5只雄性和5只雌性白羽鸡的八个组织（下丘脑、肝脏、心脏、胸大肌、腓肠肌、腹部脂肪、锁骨脂肪、皮下脂肪）中八个脂质代谢相关基因（脂肪酸合酶、酰基甘油-3-磷酸O-酰基转移酶9、过氧化物酶体增殖物激活受体、脂蛋白脂肪酶、肉碱棕榈酰转移酶1A、肉碱棕榈酰转移酶1B、酰基辅酶A脱氢酶长链、单酰甘油脂肪酶）的表达。对于脂肪酸合酶和酰基甘油-3-磷酸O-酰基转移酶9，性别之间没有差异，组织与性别的交互作用也不存在。在这两种情况下，肝脏中的表达均高于其他组织。当组织与性别的交互作用显著时，雌性下丘脑、肝脏和胸大肌中的脂肪酸合酶mRNA丰度高于雄性。对于过氧化物酶体增殖物激活受体，任何组织均不存在性别二态性。脂肪库的总体值高于下丘脑和肝脏，肌肉的值介于两者之间。雌性胸大肌和腹部脂肪中的脂蛋白脂肪酶mRNA丰度高于雄性，心脏和皮下脂肪则相反。雌性腓肠肌和锁骨脂肪中的肉碱棕榈酰转移酶1A mRNA丰度高于雄性，肝脏则相反。雌性下丘脑、肝脏和腓肠肌中的肉碱棕榈酰转移酶1B mRNA丰度高于雄性，胸大肌中的则低于雄性。结果表明，成熟鸡中脂质代谢相关基因的表达因基因和组织的不同而存在性别差异。
