Lee Siheun, Vu Hung M, Lee Jung-Hyun, Lim Heejin, Kim Min-Sik
School of Undergraduate Studies, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea.
Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea.
Biology (Basel). 2023 Mar 2;12(3):395. doi: 10.3390/biology12030395.
Technological developments and improvements in single-cell isolation and analytical platforms allow for advanced molecular profiling at the single-cell level, which reveals cell-to-cell variation within the admixture cells in complex biological or clinical systems. This helps to understand the cellular heterogeneity of normal or diseased tissues and organs. However, most studies focused on the analysis of nucleic acids (e.g., DNA and RNA) and mass spectrometry (MS)-based analysis for proteins and metabolites of a single cell lagged until recently. Undoubtedly, MS-based single-cell analysis will provide a deeper insight into cellular mechanisms related to health and disease. This review summarizes recent advances in MS-based single-cell analysis methods and their applications in biology and medicine.
单细胞分离和分析平台的技术发展与改进使得在单细胞水平上进行先进的分子分析成为可能，这揭示了复杂生物或临床系统中混合细胞内的细胞间差异。这有助于理解正常或患病组织及器官的细胞异质性。然而，直到最近，大多数研究仍集中在核酸（如DNA和RNA）分析以及基于质谱（MS）的单细胞蛋白质和代谢物分析上。毫无疑问，基于MS的单细胞分析将为与健康和疾病相关的细胞机制提供更深入的见解。本综述总结了基于MS的单细胞分析方法的最新进展及其在生物学和医学中的应用。
