Xiong Cen, Ling Liansheng
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
J Nanosci Nanotechnol. 2013 Feb;13(2):1406-10. doi: 10.1166/jnn.2013.6101.
A localized surface plasmon resonance (LSPR) light-scattering sensor for Hg2+ was developed with unmodified gold nanoparticles (AuNPs) based upon the specific recognition property of Hg2+ with T-T mismatched base pair. Oligonucleotide 5'-GTTCTTTGTCTTCA-3'(oligo-1) and 5'-TGTAGTCTATGTAC-3'(oligo-2) can adsorb on the surface of AuNPs, which can prevent them from aggregation because of electrostatic repulsion. However, the DNA hybridization occurred between oligo-1 and oligo-2 upon addition of Hg2+, can induce the desorption of oligonucleotide from the surface of AuNPs, and trigger the aggregation of AuNPs accompany with the increase of LSPR light-scattering intensity. Under the optimum conditions, the intensity was proportional to the concentration of Hg2+ over the range 53.1-530 nM, and the detection limit was 29.4 nM.
基于Hg2+与T-T错配碱基对的特异性识别特性，利用未修饰的金纳米颗粒（AuNPs）开发了一种用于Hg2+的局域表面等离子体共振（LSPR）光散射传感器。寡核苷酸5'-GTTCTTTGTCTTCA-3'（oligo-1）和5'-TGTAGTCTATGTAC-3'（oligo-2）可以吸附在AuNPs表面，由于静电排斥作用，可防止它们聚集。然而，加入Hg2+后，oligo-1和oligo-2之间发生DNA杂交，可诱导寡核苷酸从AuNPs表面解吸，并引发AuNPs聚集，同时LSPR光散射强度增加。在最佳条件下，强度与53.1-530 nM范围内的Hg2+浓度成正比，检测限为29.4 nM。
