School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Analyst. 2010 Oct;135(10):2682-7. doi: 10.1039/c0an00201a. Epub 2010 Sep 3.
A novel localized surface plasmon resonance (LSPR) light-scattering sensor for Ag(+) was developed with unmodified gold nanoparticles (AuNPs), based upon the specific recognition property of Ag(+) with a cytosine-cytosine mismatched base pair. The addition of Ag(+) induced the oligonucleotide 5'-TAC ATA CAT ACT ATC TAT CTA-3' to be desorbed from the surface of the AuNPs, resulting in the aggregation of AuNPs, accompanied by a dramatic enhancement of the LSPR light-scattering intensity. The enhancement of LSPR light-scattering intensity was proportional to the concentration of Ag(+) in the range of 0.13-1.12 μM, with a limit of detection of 62.0 nM. The results were also proved by a colorimetric method. Furthermore, this method can provide satisfactory results for the determination of Ag(+) in water samples and industrial products.
一种新型的局域表面等离子体共振（LSPR）光散射传感器用于 Ag(+)的开发与未修饰的金纳米粒子（AuNPs），基于 Ag(+)与胞嘧啶-胞嘧啶错配碱基对的特异性识别特性。Ag(+)的加入诱导寡核苷酸 5'-TAC ATA CAT ACT ATC TAT CTA-3'从 AuNPs 的表面解吸，导致 AuNPs 的聚集，同时 LSPR 光散射强度急剧增强。LSPR 光散射强度的增强与 Ag(+)的浓度在 0.13-1.12 μM 的范围内成正比，检测限为 62.0 nM。通过比色法也证明了这一结果。此外，该方法可以为水样和工业产品中 Ag(+)的测定提供令人满意的结果。
