采用高效液相色谱法和快原子轰击质谱法测定家禽胆汁中的胆汁酸谱。

Biliary bile acid profiles of domestic fowl as determined by high performance liquid chromatography and fast atom bombardment mass spectrometry.

Elkin R G, Wood K V, Hagey L R

Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.

Comp Biochem Physiol B. 1990;96(1):157-61. doi: 10.1016/0305-0491(90)90356-x.

Abstract

The biliary bile acid profiles of domestic chickens (Gallus domesticus), turkeys (Meleagris gallopavo), and ducks (Anas platyrhynchos) were determined by high performance liquid chromatography (HPLC) and fast atom bombardment mass spectrometry (FAB-MS). 2. Chenodeoxycholyltaurine and cholyltaurine were the predominant bile acids in chicken and turkey bile, whereas duck bile contained primarily chenodeoxycholyltaurine and phocaecholyltaurine. 3. Allocholyltaurine was also detected in chicken and turkey bile, but not in duck bile. 4. FAB-MS analyses of individual HPLC peak fractions from chicken and duck bile extracts confirmed the presence of either taurine-conjugated dihydroxy- or trihydroxycholanoates. 5. Direct FAB-MS analyses of avian bile extracts not subjected to HPLC permitted a rapid assessment of the relative proportion of taurine-conjugated dihydroxy- to trihydroxycholanoates.

摘要

通过高效液相色谱法（HPLC）和快原子轰击质谱法（FAB-MS）测定了家鸡（原鸡）、火鸡（吐绶鸡）和鸭（绿头鸭）的胆汁胆汁酸谱。2. 鹅去氧胆酰牛磺酸和胆酰牛磺酸是鸡和火鸡胆汁中的主要胆汁酸，而鸭胆汁主要含有鹅去氧胆酰牛磺酸和海豹胆酰牛磺酸。3. 在鸡和火鸡胆汁中也检测到了别胆酰牛磺酸，但鸭胆汁中未检测到。4. 对鸡和鸭胆汁提取物中各个HPLC峰馏分的FAB-MS分析证实了牛磺酸共轭二羟基或三羟基胆烷酸酯的存在。5. 对未经HPLC处理的禽胆汁提取物进行直接FAB-MS分析，可以快速评估牛磺酸共轭二羟基与三羟基胆烷酸酯的相对比例。