Zhou Shuai, Liu Chunxiu, Song Yilin, Cai Xinxia
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100190, China.
J Nanosci Nanotechnol. 2013 Feb;13(2):1598-601. doi: 10.1166/jnn.2013.6077.
The present study concerns methodological issue of electrochemical recordings using Nafion-coated microelectrode arrays fabricated by MEMS technology for voltammetric and amperometric measurements of dopamine (DA). The properties of Nafion-coated different temperature dried microelectrodes were determined by cyclic voltammetry. The electrochemical characteristics of the 120 degrees C dried Nafion-coated microelectrode for measures of dopamine and ascorbic acid (AA) were studied by chronoamperometry. These studies showed that the 120 degrees C dried Nafion-coated microelectrode had better recording properties: enlarged electrochemical signal for DA and high selectivity for DA versus AA. The linear response was obtained in the range of 0.1 to 50 microM with a low detection limit. The modified microelectrode arrays providing 30 microm diameter multiple sites showed the capability to simultaneously detect DA from cells, neurons and brain slices.
本研究关注使用通过微机电系统（MEMS）技术制造的涂有Nafion的微电极阵列进行电化学记录的方法学问题，用于多巴胺（DA）的伏安法和安培法测量。通过循环伏安法测定了不同温度干燥的涂有Nafion的微电极的性能。通过计时电流法研究了120℃干燥的涂有Nafion的微电极用于测量多巴胺和抗坏血酸（AA）的电化学特性。这些研究表明，120℃干燥的涂有Nafion的微电极具有更好的记录性能：DA的电化学信号增强，对DA相对于AA具有高选择性。在0.1至50 microM范围内获得线性响应，检测限较低。提供30微米直径多个位点的修饰微电极阵列显示出能够同时检测来自细胞、神经元和脑切片的DA。
