Lee Joo-Kyung, Cho Si-Hyeong, Lee Jinyoung, Lee Jung-Hwan, Kim Ah Young, Park Bae Ho, Park Jin-Goo, Busnaina Ahmed, Lee Hea Yeon
Division of Quantum Phases and Devices, Konkuk University, Seoul 143-701, South Korea.
J Nanosci Nanotechnol. 2013 Jan;13(1):139-43. doi: 10.1166/jnn.2013.6865.
The rapid identification and verification of single nucleotide polymorphisms (SNPs) were demonstrated using a well array sensor containing anti-biofouling titanium (Ti). Probe single-stranded DNA (ssDNA) was immobilized inside a titanium-well array on amine-modified glass surfaces with anti-biofouling behavior via a streptavidin-biotin interaction. Fluorescence intensity changes originating from the hybridization of nucleic acids to protein-bound nucleic acids linked to Alexa Fluor (FL) 647 were observed. The protocol was highly sensitive and reproducible for the detection of DNA hybridization. Significant changes in fluorescence signals were observed when using target DNA with a single base mismatch, indicating that this method is applicable to SNP detection. The microarray technology for the detection of SNPs using anti-biofouling Ti and other methods can be used as a highly sensitive in vitro medical sensor, as highlighted by an increase in genotyping accuracy.
使用含有抗生物污损钛（Ti）的微孔阵列传感器，展示了单核苷酸多态性（SNP）的快速鉴定和验证。通过链霉亲和素 - 生物素相互作用，将探针单链DNA（ssDNA）固定在具有抗生物污损行为的胺修饰玻璃表面的钛微孔阵列内。观察到核酸与连接到Alexa Fluor（FL）647的蛋白质结合核酸杂交产生的荧光强度变化。该方案对DNA杂交检测具有高灵敏度和可重复性。当使用具有单个碱基错配的靶DNA时，观察到荧光信号的显著变化，表明该方法适用于SNP检测。使用抗生物污损Ti检测SNP的微阵列技术和其他方法可作为高度灵敏的体外医学传感器，基因分型准确性的提高突出了这一点。
