Park Dae Keun, Park Hyung Ju, Lee Cho Yeon, Hong Daewha, Lee Young, Choi Insung S, Yun Wan Soo
Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Korea.
J Nanosci Nanotechnol. 2012 Jul;12(7):5155-9. doi: 10.1166/jnn.2012.6382.
PNA chips for the detection of the genetic polymorphism of Cytochrome P450 2C19 (CYP2C19), a well-known enzyme related to the metabolism of therapeutic drugs, were electrically-interfaced with interdigitated nanogap electrodes (INEs). The average gap distance and effective length of the INEs were about approximately 70 nm and approximately 140/m, respectively. Those INEs having the aspect ratio of about 2000, were prepared by the combination of the photolithography (for the formation of initial electrodes) and the surface-catalyzed chemical deposition (for the gap narrowing), without the e-beam lithography. The PNA probes for the detection of CYP2C19 were immobilized in the gap region of INEs via Schiff base formation. The I-V characteristics clearly showed a sharp increase in the conductance between the nanogap electrodes upon the PNA-DNA hybridization, followed by the adsoprtion of functionalized Au nanoparticles. Four different target DNAs for the diagnosis of CYP2C19 polymorphism were successfully detected and discriminated with the INE-based PNA chips.
用于检测细胞色素P450 2C19（CYP2C19）基因多态性的肽核酸（PNA）芯片与叉指式纳米间隙电极（INE）进行了电连接，CYP2C19是一种与治疗药物代谢相关的著名酶。INE的平均间隙距离和有效长度分别约为70纳米和约140微米。那些纵横比约为2000的INE是通过光刻（用于形成初始电极）和表面催化化学沉积（用于间隙缩小）相结合制备的，无需电子束光刻。用于检测CYP2C19的PNA探针通过席夫碱形成固定在INE的间隙区域。电流-电压特性清楚地表明，在PNA-DNA杂交后，纳米间隙电极之间的电导急剧增加，随后是功能化金纳米颗粒的吸附。基于INE的PNA芯片成功检测并区分了四种用于诊断CYP2C19多态性的不同目标DNA。
