Tropical Marine Biological Resources Research Center, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, People's Republic of China.
Can J Microbiol. 2013 May;59(5):324-32. doi: 10.1139/cjm-2012-0735. Epub 2013 Mar 12.
Bacterial community diversity and the effects of environmental factors on bacterial community composition during 2 spring phytoplankton blooms in the central Yellow Sea were investigated by using denaturing gradient gel electrophoresis (DGGE) and multivariate statistical analysis. The Shannon-Weaver indices (H') of bacterial diversity from samples at station B23 were higher than those at station B20. Cluster analysis based on DGGE band patterns indicated temporal variations of bacterial community at the 2 bloom stations but a vertical distribution pattern only at station B20. The predominant bacterial groups were affiliated with Alphaproteobacteria, Gammaproteobacteria, Cytophaga-Flavobacterium-Bacteroides, Deltaproteobacteria, and Actinobacteria. The effects of environmental factors on bacterial community were analyzed by canonical correspondence analysis. Bacterial community structures were significantly affected by silicate at station B20 and by Paralia sulcata and Heterocapsa spp. at station B23. From the results, phytoplankton species composition had a significant effect on bacterial community structure during phytoplankton blooms in the central Yellow Sea.
采用变性梯度凝胶电泳（DGGE）和多元统计分析方法，研究了黄海中部 2 次春季浮游植物水华期间细菌群落多样性以及环境因子对细菌群落组成的影响。B23 站位样品的细菌多样性 Shannon-Weaver 指数（H'）高于 B20 站位。基于 DGGE 条带模式的聚类分析表明，2 个水华站的细菌群落存在时间变化，但仅在 B20 站位存在垂直分布模式。主要细菌类群与α变形菌、γ变形菌、噬纤维菌-黄杆菌-拟杆菌、δ变形菌和放线菌有关。通过典范对应分析分析了环境因子对细菌群落的影响。B20 站位的硅和 Paralia sulcata 和 Heterocapsa spp.对细菌群落结构有显著影响，B23 站位。结果表明，浮游植物种类组成对黄海中部浮游植物水华期间的细菌群落结构有显著影响。
