Atmosphere and Ocean Research Institute, The University of Tokyo, Kashiwa, Chiba, Japan.
Microbes Environ. 2011;26(4):339-46. doi: 10.1264/jsme2.me11212. Epub 2011 Jul 27.
Using bromodeoxyuridine (BrdU) magnetic beads immunocapture and a PCR-denaturing gradient gel electrophoresis (DGGE) technique (BUMP-DGGE), we determined seasonal variations in the community structures of actively growing bacteria in the neritic waters of Hiroshima Bay, western Japan. The community structures of actively growing bacteria were separated into two clusters, corresponding to the timing of phytoplankton blooms in the autumn-winter and spring-summer seasons. The trigger for changes in bacterial community structure was related to organic matter supply from phytoplankton blooms. We identified 23 phylotypes of actively growing bacteria, belonging to Alphaproteobacteria (Roseobacter group, 9 phylotypes), Gammaproteobacteria (2 phylotypes), Bacteroidetes (8 phylotypes), and Actinobacteria (4 phylotypes). The Roseobacter group and Bacteroidetes were dominant in actively growing bacterial communities every month, and together accounted for more than 70% of the total DGGE bands. We revealed that community structures of actively growing bacteria shifted markedly in the wake of phytoplankton blooms in the neritic waters of Hiroshima Bay.
利用溴脱氧尿苷（BrdU）磁珠免疫捕获和聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）技术，我们测定了日本西部广岛湾近岸水域中活跃生长细菌群落的季节性变化。活跃生长细菌的群落结构分为两个聚类，分别对应于秋季-冬季和春季-夏季浮游植物的爆发时间。细菌群落结构变化的触发因素与浮游植物爆发产生的有机物供应有关。我们鉴定出了 23 个活跃生长细菌的种型，属于 α-变形菌（Roseobacter 群，9 个种型）、γ-变形菌（2 个种型）、拟杆菌门（Bacteroidetes，8 个种型）和放线菌（Actinobacteria，4 个种型）。Roseobacter 群和拟杆菌门在活跃生长细菌群落中每个月都占优势，共占 DGGE 条带总数的 70%以上。我们揭示了在日本西部广岛湾近岸水域浮游植物爆发后，活跃生长细菌的群落结构发生了明显变化。
