Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
J Dig Dis. 2013 Oct;14(10):543-51. doi: 10.1111/1751-2980.12068.
To evaluate the state of oral tolerance and its therapeutic role in mice with dextran sulfate sodium (DSS)-induced colitis.
Delayed-type hypersensitivity (DTH) was determined 7 and 14 days after DSS-induced colitis and control mice. Disease activity index (DAI) score and colonic histopathological score were measured 7 days after colonic extracted protein (CEP) or bovine serum albumin (BSA) (control) was administrated, with the evaluation of Th1-Th2 balance in the spleen, Peyer's patch and γδ T cells in intraepithelial lymphocytes and lamina proper lymphocytes in the intestine.
After fed with 250 μg ovalbumin oral tolerance was induced in 7 days in both DSS-induced colitis and control mice, while oral tolerance persisted in the control mice but vanished in DSS-induced colitis 14 days after ovalbumin challenge. DAI and colonic histopathological scores were decreased significantly after the ingestion of CEP (controlled by BSA) in DSS-induced colitis with significant reduction of Th1 and the ratio of Th1 to Th2 in Peyer's patch as well as the γδ T cells in lamina proper lymphocytes in the intestine. No significant difference in Th1-Th2 balance in the spleen and γδ T cells in intraepithelial lymphocytes in the intestine were observed.
There is a defect in oral tolerance at day 7 in DSS-induced colitis. If taken orally, CEP may have a protective role in DSS-induced colitis, which may be related to the deflection from Th1 to Th2 in Peyer's patch and the reduction of γδ T cells in lamina proper lymphocytes in the intestine.
评估葡聚糖硫酸钠（DSS）诱导结肠炎小鼠的口服耐受状态及其治疗作用。
在 DSS 诱导结肠炎后 7 天和 14 天，测定迟发型超敏反应（DTH）和对照小鼠。在给予结肠提取蛋白（CEP）或牛血清白蛋白（BSA）（对照）后 7 天，测定疾病活动指数（DAI）评分和结肠组织病理学评分，并评估脾、派尔集合淋巴结和上皮内淋巴细胞中的 γδ T 细胞以及固有层淋巴细胞中的 Th1-Th2 平衡。
在给予 250μg 卵清蛋白后，7 天内 DSS 诱导结肠炎和对照小鼠均诱导口服耐受，但在卵清蛋白挑战后 14 天，对照小鼠的口服耐受持续存在，而 DSS 诱导结肠炎小鼠的口服耐受消失。在 DSS 诱导结肠炎小鼠中，口服 CEP（以 BSA 为对照）可显著降低 DAI 和结肠组织病理学评分，同时显著降低派尔集合淋巴结中的 Th1 和 Th1/Th2 比值以及固有层淋巴细胞中的 γδ T 细胞。脾和肠上皮内淋巴细胞中的 Th1-Th2 平衡无显著差异。
DSS 诱导结肠炎在第 7 天存在口服耐受缺陷。如果口服给予 CEP，可能对 DSS 诱导结肠炎具有保护作用，这可能与派尔集合淋巴结中 Th1 向 Th2 的偏向以及固有层淋巴细胞中 γδ T 细胞的减少有关。
