Sang Li-Xuan, Chang Bing, Zhu Jun-Feng, Yang Fang-Li, Li Yan, Jiang Xue-Feng, Wang Da-Nan, Lu Chang-Long, Sun Xun
Li-Xuan Sang, Jun-Feng Zhu, Fang-Li Yang, Yan Li, Xue-Feng Jiang, Da-Nan Wang, Chang-Long Lu, Xun Sun, Department of Immunology, China Medical University, Shenyang 110122, Liaoning Province, China.
World J Gastroenterol. 2017 Jun 7;23(21):3850-3863. doi: 10.3748/wjg.v23.i21.3850.
To assess the effect of sodium selenite on the severity of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice.
Mice were randomly divided into four groups ( = 10/group): normal group, selenium (Se) group, chronic colitis group, and Se + chronic colitis group. The mice were sacrificed on day 26. Survival rates, clinical symptoms, colon length, and histological changes were determined. The percentages and absolute numbers of immune system cells in the lamina propria lymphocytes (LPL) of the colon, the expression of mRNA in colon tissue, and the concentrations of Th1, Th17, and Treg cytokines in LPL from the large intestine, were measured.
Se significantly ameliorated the symptoms of colitis and histological injury ( < 0.05 each), increasing the proportions of neutrophils and CD4 CD25 T cells ( < 0.05 each) and decreasing the proportions of γδT cells, CD4, CD4CD44, and CD4 CD69 T cells in LPL ( < 0.05 each). Moreover, Se reduced the expression of IL-6, IFN-γ, IL-17A, IL-21, T-bet, and RORγt ( < 0.05 each), but enhanced the expression of IL-10 and Foxp3 ( < 0.05 each).
These results suggest that Se protects against DSS-induced chronic colitis perhaps by increasing the number of CD4(+)CD25(+) Tregs that suppress the secretion of proinflammatory cytokines and populations of Th1, Th17, and γδT cells.
评估亚硒酸钠对葡聚糖硫酸钠（DSS）诱导的C57BL/6小鼠结肠炎严重程度的影响。
将小鼠随机分为四组（每组n = 10）：正常组、硒（Se）组、慢性结肠炎组和Se +慢性结肠炎组。在第26天处死小鼠。测定生存率、临床症状、结肠长度和组织学变化。测量结肠固有层淋巴细胞（LPL）中免疫系统细胞的百分比和绝对数量、结肠组织中mRNA的表达以及大肠LPL中Th1、Th17和Treg细胞因子的浓度。
硒显著改善了结肠炎症状和组织学损伤（均P < 0.05），增加了中性粒细胞和CD4⁺CD25⁺T细胞的比例（均P < 0.05），并降低了LPL中γδT细胞、CD4⁺、CD4⁺CD44⁺和CD4⁺CD69⁺T细胞的比例（均P < 0.05）。此外，硒降低了IL-6、IFN-γ、IL-17A、IL-21、T-bet和RORγt的表达（均P < 0.05），但增强了IL-10和Foxp3的表达（均P < 0.05）。
这些结果表明，硒可能通过增加抑制促炎细胞因子分泌的CD4⁺CD25⁺调节性T细胞数量以及Th1、Th17和γδT细胞群体，来预防DSS诱导的慢性结肠炎。
