Hairul Bahara Nur Hidayah, Tye Gee Jun, Choong Yee Siew, Ong Eugene Boon Beng, Ismail Asma, Lim Theam Soon
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Biologicals. 2013 Jul;41(4):209-16. doi: 10.1016/j.biologicals.2013.04.001. Epub 2013 May 3.
With major developments in molecular biology, numerous display technologies have been successfully introduced for recombinant antibody production. Even so, phage display still remains the gold standard for recombinant antibody production. Its success is mainly attributed to the robust nature of phage particles allowing for automation and adaptation to modifications. The generation of monospecific binders provides a vital tool for diagnostics at a lower cost and higher efficiency. The flexibility to modify recombinant antibodies allows great applicability to various platforms for use. This review presents phage display technology, application and modifications of recombinant antibodies for diagnostics.
随着分子生物学的重大发展，许多展示技术已成功应用于重组抗体的生产。即便如此，噬菌体展示仍然是重组抗体生产的金标准。其成功主要归因于噬菌体颗粒的强大特性，使其能够实现自动化并适应修饰。单特异性结合物的产生为诊断提供了一种低成本、高效率的重要工具。重组抗体的修饰灵活性使其在各种应用平台上具有广泛的适用性。本文综述了噬菌体展示技术、重组抗体在诊断中的应用及修饰。
