应用 SXP 特异性重组单克隆抗体建立班氏丝虫病抗原检测 ELISA 方法。
Development of an Antigen Detection ELISA for Bancroftian Filariasis Using SXP-Specific Recombinant Monoclonal Antibody.
机构信息
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia.
出版信息
Am J Trop Med Hyg. 2019 Aug;101(2):436-440. doi: 10.4269/ajtmh.19-0034.
Lymphatic filariasis is a mosquito-borne parasitic disease responsible for morbidity and disability that affects 1.2 billion people worldwide, mainly the poor communities. Currently, filarial antigen testing is the method of choice for the detection of bancroftian filariasis, and to date, there are two commonly used tests. In the present study, a recently reported recombinant monoclonal antibody (5B) specific to SXP filarial antigen was used in developing an ELISA for the detection of circulating filarial antigen in sera of patients with bancroftian filariasis. The performance of the ELISA was evaluated using 124 serum samples. The ELISA was positive with all sera from microfilaremic bancroftian filariasis patients ( = 34). It also showed 100% diagnostic specificity when tested with sera from 50 healthy individuals and 40 patients with other parasitic diseases. The developed assay using the novel 5B recombinant monoclonal antibody could potentially be a promising alternative antigen detection test for bancroftian filariasis.
淋巴丝虫病是一种由蚊子传播的寄生虫病，可导致全球 12 亿人患病和残疾，主要是贫困社区。目前，丝虫抗原检测是检测班氏丝虫病的首选方法，迄今为止，有两种常用的检测方法。在本研究中，使用最近报道的针对 SXP 丝虫抗原的重组单克隆抗体 (5B) 来开发一种用于检测班氏丝虫病患者血清中循环丝虫抗原的 ELISA。使用 124 份血清样本评估 ELISA 的性能。该 ELISA 对所有微丝蚴血症的班氏丝虫病患者血清均呈阳性（n=34）。当用 50 名健康个体和 40 名患有其他寄生虫病的患者的血清进行测试时，该 ELISA 也显示出 100%的诊断特异性。使用新型 5B 重组单克隆抗体开发的检测方法可能是一种有前途的班氏丝虫病抗原检测替代方法。
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