Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd. Kansas City, KS 66160, United States.
Virology. 2013 Jul 20;442(1):82-96. doi: 10.1016/j.virol.2013.04.002. Epub 2013 May 4.
Rhesus macaque APOBEC3A (rhA3A) is capable of restricting both simian-human immunodeficiency virus (SHIVΔvif) and human immunodeficiency virus (HIV-1Δvif) to a greater extent than hA3A. We constructed chimeric A3A proteins to define the domains required for differential lentivirus restriction. Substitution of amino acids 25-33 from rhA3A into hA3A was sufficient to restrict HIVΔvif to levels similar to rhA3A restriction of SHIVΔvif. We tested if differential lentivirus restriction is conserved between A3A from Old World monkey and hominid lineages. A3A from African green monkey restricted SHIVΔvif but not HIV-1Δvif and colobus monkey A3A restricted both wild type and SHIVΔvif and HIV-1Δvif. In contrast, the gibbon ape A3A restricted neither SHIVΔvif nor HIV-1Δvif. Restriction of SHIVΔvif and HIV-1Δvif by New World monkey A3A proteins was not conserved as the A3A from the squirrel monkey but not the northern owl monkey restricted SHIVΔvif. Finally, the colobus A3A protein appears to restrict by a novel post-entry mechanism.
食蟹猴 APOBEC3A（rhA3A）比 hA3A 更能限制猴免疫缺陷病毒（SHIVΔvif）和人类免疫缺陷病毒（HIV-1Δvif）。我们构建了嵌合 A3A 蛋白，以确定限制不同慢病毒所需的结构域。将 rhA3A 中的氨基酸 25-33 替换为 hA3A，足以将 HIVΔvif 限制在类似于 rhA3A 限制 SHIVΔvif 的水平。我们测试了 A3A 之间的差异慢病毒限制是否在旧世界猴和人类谱系之间保守。来自非洲绿猴的 A3A 限制了 SHIVΔvif，但不限制 HIV-1Δvif，而疣猴的 A3A 限制了野生型和 SHIVΔvif 和 HIV-1Δvif。相比之下，长臂猿的 A3A 既不限制 SHIVΔvif 也不限制 HIV-1Δvif。新的世界猴 A3A 蛋白对 SHIVΔvif 和 HIV-1Δvif 的限制并不保守，因为来自松鼠猴的 A3A 而不是北方夜猴限制了 SHIVΔvif。最后，疣猴 A3A 蛋白似乎通过一种新的进入后机制来限制。
