Division of Breast Cancer Research, Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK.
J Mammary Gland Biol Neoplasia. 2013 Jun;18(2):227-31. doi: 10.1007/s10911-013-9285-5. Epub 2013 May 7.
Whole-mount immunofluorescent staining facilitates the profiling of protein expression patterns within diverse and complex tissues. Thanks to the application of antibodies on whole mounted instead of sectioned specimens, this technique has many advantages with respect to the preservation of biological and pathological features of specimens when compared to conventional immunohistological methods. Here, we describe a protocol and optimal conditions of whole-mount immunofluorescence for studying the formation of mammary primordia. We also show an example three-dimensional reconstruction of a mammary primordium based on z-stacked images of a whole-mount stained specimen using confocal microscopy and image analysis software.
全组织免疫荧光染色有助于在不同且复杂的组织中分析蛋白质表达模式。与传统免疫组织化学方法相比，由于该技术将抗体应用于整个组织而不是切片标本上，因此在保留标本的生物学和病理学特征方面具有许多优势。在此，我们描述了一种用于研究乳腺原基形成的全组织免疫荧光技术的方案和最佳条件。我们还展示了基于共聚焦显微镜和图像分析软件对全组织染色标本的 z 堆叠图像进行三维重建的示例。
