Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2021;2214:143-155. doi: 10.1007/978-1-0716-0958-3_10.
Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the sample processing, protein detection using antibodies and confocal imaging of isolated preimplantation to early postimplantation mouse embryos up to Embryonic day 8.0 (E8.0).
免疫荧光染色可使蛋白质表达在细胞甚至亚核水平可视化。全胚胎染色保留了生物样本的三维空间信息，从而可以全面解释表达域。本文描述了从植入前到早期植入后（E8.0）的 8.0 天的分离小鼠胚胎的样本处理、抗体蛋白检测和共聚焦成像。
