Chen Yushu, Bai Bo, Zhang Shujiang, Ye Jing, Zhai Haohan, Chen Yi, Zhang Linlin, Zeng Yanjun
Department of Orthopedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, 1st Affiliated Hospital of Guangzhou Medical University, No.151 Yanjiang Road, Guangzhou, 510120, People's Republic of China.
J Biomed Mater Res A. 2014 May;102(5):1294-304. doi: 10.1002/jbm.a.34788. Epub 2013 Jun 7.
Both decalcified bone matrix (DBM) and fibrin gel possess good biocompatibility, so they are used as scaffolds to culture bone marrow mesenchymal stem cells (BMSCs). The feasibility and efficacy of using compound material being made of decalcified bone matrix and fibrin gel as a three-dimensional scaffold for bone growth were investigated. BMSCs were isolated from the femur of rabbit, then seeded in prepared scaffolds after incubation for 28 days in vitro. In vivo: 30 New Zealand White Rabbits received bone defect in left radius and divided three treatment groups randomly: (1) BMSCs/decalcified bone matrix/fibrin glue as experimental group; (2) decalcified bone matrix/fibrin glue without cells as control group; (3) nothing was implanted into the bone defects as blank group. The observation period of specimens was 12 weeks, and were analyzed bone formation in terms of serum proteomics (2D-PAGE and MALDI-TOF-TOF-MS), hematoxylin-eosin (HE) staining, ALP staining, and Osteopontin immunofluorescence detection. The experimental group present in three peculiar kinds of proteins, whose Geninfo identifier (GI) number were 136466, 126722803, and 126723746, respectively, correspond to TTR protein, ALB protein, RBP4 protein, and the histological inspections were superior to the other group. The content of osteopontin in experimental group was significantly higher than control group (p < 0.05). The overall results indicated that a combined material being made of BMSCs/decalcified bone matrix/fibrin glue can result in successful bone formation and decalcified bone matrix/fibrin glue admixtures can be used as a scaffold for bone tissue engineering.
脱钙骨基质（DBM）和纤维蛋白凝胶都具有良好的生物相容性，因此它们被用作培养骨髓间充质干细胞（BMSCs）的支架。研究了使用由脱钙骨基质和纤维蛋白凝胶制成的复合材料作为骨生长三维支架的可行性和有效性。从兔股骨中分离出BMSCs，体外培养28天后接种到制备好的支架上。体内实验：30只新西兰白兔左侧桡骨制造骨缺损，随机分为三个治疗组：（1）BMSCs/脱钙骨基质/纤维蛋白胶作为实验组；（2）无细胞的脱钙骨基质/纤维蛋白胶作为对照组；（3）骨缺损处不植入任何东西作为空白组。标本观察期为12周，通过血清蛋白质组学（二维聚丙烯酰胺凝胶电泳和基质辅助激光解吸电离飞行时间串联质谱）、苏木精-伊红（HE）染色、碱性磷酸酶（ALP）染色和骨桥蛋白免疫荧光检测分析骨形成情况。实验组出现了三种特殊蛋白质，其基因信息标识符（GI）编号分别为136466、126722803和126723746，分别对应甲状腺素转运蛋白（TTR）、白蛋白（ALB）、视黄醇结合蛋白4（RBP4），组织学检查优于其他组。实验组骨桥蛋白含量明显高于对照组（p<0.05）。总体结果表明，由BMSCs/脱钙骨基质/纤维蛋白胶制成的复合材料可成功促进骨形成，脱钙骨基质/纤维蛋白胶混合物可作为骨组织工程的支架。
