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一种编码新型人类神经营养因子的cDNA的克隆与表达

Cloning and expression of a cDNA encoding a novel human neurotrophic factor.

作者信息

Kaisho Y, Yoshimura K, Nakahama K

机构信息

Biotechnology Research Laboratories, Takeda Chemical Industries Ltd, Osaka, Japan.

出版信息

FEBS Lett. 1990 Jun 18;266(1-2):187-91. doi: 10.1016/0014-5793(90)81536-w.

DOI:10.1016/0014-5793(90)81536-w
PMID:2365067
Abstract

A cDNA encoding a novel human neurotrophic factor (designated nerve growth factor-2; NGF-2) was cloned from a human glioma cDNA library using a synthetic DNA corresponding to human nerve growth factor (NGF). The cloned cDNA encodes a polypeptide composed of 257 amino acid residues including a prepro-sequence of 138 residues and a mature region of 119 residues. The amino acid sequence of human NGF-2 exhibits 58% similarity with that of human NGF. Conditioned medium of COS-7 cells transfected with an expression plasmid for human NGF-2 cDNA supported the survival of sensory neurons isolated from dorsal root ganglia of embryonic chicks. A 1.5 kb of NGF-2 mRNA can be detected from an early development stage in rat brain, by Northern blotting analysis.

摘要

利用对应于人神经生长因子（NGF）的合成DNA，从人胶质瘤cDNA文库中克隆出一个编码新型人类神经营养因子（命名为神经生长因子-2；NGF-2）的cDNA。克隆的cDNA编码一个由257个氨基酸残基组成的多肽，包括一个138个残基的前原序列和一个119个残基的成熟区域。人NGF-2的氨基酸序列与人类NGF的氨基酸序列具有58%的相似性。用人类NGF-2 cDNA表达质粒转染的COS-7细胞的条件培养基支持从胚胎鸡背根神经节分离的感觉神经元的存活。通过Northern印迹分析，在大鼠脑的早期发育阶段可检测到1.5kb的NGF-2 mRNA。

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