Iaguzhinskiĭ L S, Motovilov K A, Volkov E M, Eremeev S A
Biofizika. 2013 Jan-Feb;58(1):117-25.
In the process of mitochondrial respiratory H(+)-pumps functioning, the fraction membrane-bound protons (R-protons), which have an excess of free energy is formed. According to R.J. Williams this fraction is included as energy source in the reaction of ATP synthesis. Previously, in our laboratory was found the formation of this fraction was found in the mitochondria and on the outer surface of mitoplast. On the mitoslast model we strictly shown that non-equilibrium R-proton fraction is localized on the surface of the inner mitochondrial membrane. In this paper a surface-active compound--anion of 2,4,6-trichloro-3-pentadecylphenol (TCP-C15) is described, which selectively interacts with the R-protons fraction in mitochondria. A detailed description of the specific interaction of the TCP-C15 with R-protons fraction in mitochondria is presented. Moreover, in this work it was found that phosphate transport system reacts with the R-protons fraction in mitochondria and plays the role of the endogenous volume regulation system of this fraction. The results of experiments are discussed in the terms of a local coupling model of the phosphorylation mechanism.
在线粒体呼吸性H(+)泵功能发挥过程中，会形成具有过量自由能的膜结合质子部分（R-质子）。根据R.J.威廉姆斯的理论，这一部分作为能量来源参与ATP合成反应。此前，我们实验室发现线粒体及线粒体质外膜表面会形成这一部分。在mitoslast模型上，我们严格证明了非平衡R-质子部分定位于线粒体内膜表面。本文描述了一种表面活性化合物——2,4,6-三氯-3-十五烷基苯酚（TCP-C15）的阴离子，它能与线粒体中的R-质子部分选择性相互作用。文中详细介绍了TCP-C15与线粒体中R-质子部分的特异性相互作用。此外，在这项研究中还发现，磷酸盐转运系统与线粒体中的R-质子部分发生反应，并充当该部分内源性体积调节系统的角色。实验结果依据磷酸化机制的局部偶联模型进行了讨论。
