Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
Analyst. 2013 Jun 21;138(12):3478-82. doi: 10.1039/c3an36914e. Epub 2013 May 8.
We developed a gold-nanopatterned immunoassay chip for single-molecule detection of interleukin-6 based on evanescent field-enhanced fluorescence imaging. The detection limit (496 zM) means that only four molecules need to be present per spot for detection. The sensitivity of this chip is ~5.4 × 10(4)-fold greater than that of a commercial ELISA for IL-6.
我们开发了一种基于倏逝场增强荧光成像的金纳米图案免疫分析芯片，用于白细胞介素-6 的单分子检测。检测限（496 zM）意味着每个斑点只需存在四个分子即可进行检测。该芯片的灵敏度比商用 IL-6 ELISA 高约 5.4×10(4)倍。
