SRI International Inc., Menlo Park, CA, USA.
J Anal Toxicol. 2013 Jul-Aug;37(6):382-5. doi: 10.1093/jat/bkt037. Epub 2013 May 6.
Most hospital laboratories do not measure blood cyanide concentrations, and samples must be sent to reference laboratories. A simple method is needed for measuring cyanide in hospitals. The authors previously developed a method to quantify cyanide based on the high binding affinity of the vitamin B12 analog, cobinamide, for cyanide and a major spectral change observed for cyanide-bound cobinamide. This method is now validated in human blood, and the findings include a mean inter-assay accuracy of 99.1%, precision of 8.75% and a lower limit of quantification of 3.27 µM cyanide. The method was applied to blood samples from children treated with sodium nitroprusside and it yielded measurable results in 88 of 172 samples (51%), whereas the reference laboratory yielded results in only 19 samples (11%). In all 19 samples, the cobinamide-based method also yielded measurable results. The two methods showed reasonable agreement when analyzed by linear regression, but not when analyzed by a standard error of the estimate or paired t-test. Differences in results between the two methods may be because samples were assayed at different times on different sample types. The cobinamide-based method is applicable to human blood, and can be used in hospital laboratories and emergency rooms.
大多数医院实验室不测量血液氰化物浓度，必须将样本送到参考实验室。医院需要一种简单的方法来测量氰化物。作者之前开发了一种基于维生素 B12 类似物 cobinamide 与氰化物的高结合亲和力以及观察到的氰化物结合 cobinamide 的主要光谱变化来定量氰化物的方法。该方法现已在人血中得到验证，研究结果包括平均批内准确度为 99.1%、精密度为 8.75%和定量下限为 3.27µM 氰化物。该方法应用于接受硝普钠治疗的儿童的血液样本，在 172 个样本中有 88 个（51%）可获得可测量的结果，而参考实验室仅在 19 个样本（11%）中获得结果。在所有 19 个样本中，基于 cobinamide 的方法也可获得可测量的结果。两种方法通过线性回归分析显示出合理的一致性，但通过估计标准误差或配对 t 检验分析则不一致。两种方法之间的结果差异可能是因为样本在不同时间、不同类型的样本上进行了检测。基于 cobinamide 的方法适用于人血，可以在医院实验室和急诊室使用。
