Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand.
J Sci Food Agric. 2013 Oct;93(13):3390-4. doi: 10.1002/jsfa.6217. Epub 2013 Jun 27.
The potential utilization of viscera and combs, by-products from the chicken-processing industry, was investigated. Chicken combs have been reported to consist of hyaluronic acid (HA) bound to protein. Thus proteases extracted from broiler viscera were applied to digest combs to separate HA, a high-value ingredient widely used in pharmaceutical and medical products.
The highest activity of crude protease extracted from chicken intestine and pancreas was 0.35 U mg(-1) enzyme at 60 °C and pH 7.5. pH stability of the enzyme was attained from pH 6 to 8, while its thermal stability declined from 30 to 50 °C, with complete activity loss occurring after 30 min at temperatures above 60 °C. Therefore the optimal conditions for broiler comb digestion by crude protease in this study were chosen as pH 7.5 and 50 °C. The results showed that the yield of crude enzyme-extracted HA was lower (P < 0.05) than that obtained by commercial papain digestion. Similar identity of extracted HA and HA standard was verified by cellulose acetate electrophoresis and Fourier transform infrared spectroscopy.
The crude enzyme extract from chicken intestine and pancreas had high proteolytic activity and could be used successfully to separate HA from broiler combs. The method provides an appropriate means to add value to poultry-processing waste.
研究了从鸡肉加工工业的副产物中利用内脏和梳子的潜力。据报道，鸡梳子由与蛋白质结合的透明质酸 (HA) 组成。因此，从肉鸡内脏中提取的蛋白酶被应用于消化梳子以分离 HA，HA 是一种广泛用于制药和医疗产品的高价值成分。
从鸡肠和胰腺中提取的粗蛋白酶的最高活性为 0.35 U mg(-1)酶，在 60°C 和 pH7.5 时。该酶的 pH 稳定性在 pH6 到 8 之间，而其热稳定性在 30 到 50°C 之间下降，在 60°C 以上温度下 30 分钟后完全失去活性。因此，本研究中粗蛋白酶消化肉鸡梳子的最佳条件选择为 pH7.5 和 50°C。结果表明，粗酶提取的 HA 产量低于（P<0.05）商用木瓜蛋白酶消化的产量。通过醋酸纤维素电泳和傅里叶变换红外光谱验证了提取的 HA 与 HA 标准品具有相似的同一性。
鸡肠和胰腺的粗酶提取物具有较高的蛋白水解活性，可成功用于从肉鸡梳子中分离 HA。该方法为家禽加工废物增值提供了一种合适的手段。
